|
| Status |
Public on May 01, 2008 |
| Title |
DBA/2J |
| Sample type |
RNA |
| |
|
| Source name |
DBA/2J
|
| Organism |
Mus musculus |
| Characteristics |
DBA/2J
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Samples were taken from epididymal fat and frozen on dry ice. Frozen tissues were individually pulverized. Total RNA was extracted from the resultant frozen tissue powder using TRI-reagent (Sigma) according to the manufacturer's recommendations. Extracted RNA was additionally purified on RNeasy mini columns (Qiagen). RNA quantity and quality was verified on Bioanalyser 2100 (Agilent). For each strain, RNA was pooled so that the final amount was 3 micrograms total RNA, with each mouse represented in equal proportions. The number of mice pooled per strain is noted under the Sample Description section.
|
| Label |
biotin
|
| Label protocol |
Three micrograms of total RNA was processed to produce biotinylated cRNA targets using standard Affymetrix procedures
|
| |
|
| Hybridization protocol |
standard Affymetrix procedures
|
| Scan protocol |
standard Affymetrix procedures
|
| Description |
epididymal fat was taken from 3 25-week old males and pooled
|
| Data processing |
gcRMA
|
| |
|
| Submission date |
Jun 05, 2007 |
| Last update date |
Jun 07, 2007 |
| Contact name |
John R Walker |
| E-mail(s) |
jwalker@gnf.org
|
| Phone |
858-812-1636
|
| Organization name |
Genomics Institute of the Novartis Research Foundation
|
| Lab |
Genetics Core
|
| Street address |
10675 John Jay Hopkins
|
| City |
San Diego |
| State/province |
CA |
| ZIP/Postal code |
92121 |
| Country |
USA |
| |
|
| Platform ID |
GPL1073 |
| Series (1) |
| GSE8028 |
Pathway analysis of expression QTL data in fat identifies a role for cyclin H in oxidative phosphorylation |
|
