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Sample GSM201765 Query DataSets for GSM201765
Status Public on Nov 01, 2007
Title RWPE cell treated with EZH2 siRNA Replicate 2
Sample type RNA
 
Channel 1
Source name Total RNA from EZH2 siRNA-treated RWPE cells labeled with Cyanine-5 (red).
Organism Homo sapiens
Characteristics RWPE
EZH2 siRNA-transfected
Age: day 2
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trizol following manufacturer's instructions
Label Cy5
Label protocol 30µg of total RNA were primed with 6 µl of poly-dT primers for 10min, then reverse-transcribed at 42°C for 1 h in the presence of 400 U SuperScript II RTase (Invitrogen), and 100 µM each dATP, dCTP, dGTP, with 25 µM dTTP, 25 µM aadUTP; the reaction mix were then cleaned and concentrated by Microcon filter, and labelled with Cy3 or Cy5 dyes at RT for 1hour
 
Channel 2
Source name Total RNA from luciferase siRNA-treated RWPE cells labeled with Cyanine-3 (green).
Organism Homo sapiens
Characteristics luciferase siRNA-transfected
Age: day 2
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trizol following manufacturer's instructions
Label Cy3
Label protocol 30µg of total RNA were primed with 6 µl of poly-dT primers for 10min, then reverse-transcribed at 42°C for 1 h in the presence of 400 U SuperScript II RTase (Invitrogen), and 100 µM each dATP, dCTP, dGTP, with 25 µM dTTP, 25 µM aadUTP; the reaction mix were then cleaned and concentrated by Microcon filter, and labelled with Cy3 or Cy5 dyes at RT for 1hour
 
 
Hybridization protocol human Cot DNA and yeast tRNA were added to labelled cDNA and hybridized in SSC/SDS buffer to arrays
Scan protocol Scanned on an Affymetrix Axon scanner
Images were quantified using Genepix6.0
Description Biological replicate 2 of 3. EZH2-siRNA-treated vs. luciferase siRNA-treated control
Data processing median normalized, background subtracted data obtained
 
Submission date Jun 15, 2007
Last update date Aug 14, 2011
Contact name Jindan Yu
E-mail(s) jindan-yu@northwestern.edu
Organization name Northwestern University
Department Medicine - Hem/Onc
Lab Yu
Street address 303 E. Superior St.
City Chicago
State/province IL
ZIP/Postal code 60611
Country USA
 
Platform ID GPL2013
Series (1)
GSE8145 human prostate and breast epithelial cell lines with EZH2 siRNA interference

Data table header descriptions
ID_REF
VALUE normalized log2 ratio (Cy5/Cy3)

Data table
ID_REF VALUE
Hs6-1-1-1 0.908
Hs6-1-2-1 0.883
Hs6-1-3-1 0.786
Hs6-1-4-1 0.938
Hs6-1-5-1 0.942
Hs6-1-6-1 1.124
Hs6-1-7-1 0.827
Hs6-1-8-1 0.85
Hs6-1-9-1 1.03
Hs6-1-10-1 0.881
Hs6-1-11-1 0.841
Hs6-1-12-1 0.738
Hs6-1-13-1 0.931
Hs6-1-14-1 0.632
Hs6-1-15-1 0.895
Hs6-1-16-1 0.657
Hs6-1-17-1 0.427
Hs6-1-18-1 0.898
Hs6-1-19-1 1.399
Hs6-1-20-1 1.524

Total number of rows: 20000

Table truncated, full table size 349 Kbytes.




Supplementary file Size Download File type/resource
GSM201765.gpr.gz 1.9 Mb (ftp)(http) GPR
Processed data included within Sample table

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