|
| Status |
Public on Oct 01, 2016 |
| Title |
differentiated_d4_replicate2 |
| Sample type |
RNA |
| |
|
| Source name |
embryonic stem cells_differentiated_d4
|
| Organism |
Mus musculus |
| Characteristics |
cell line: J1
cell type: ES
|
| Growth protocol |
Mouse J1 ES cells were cultivated in 0.1% gelatin coated dishes using DMEM (Dulbecco’s modified Eagle’s medium) supplemented with 20% fetal bovine serum (FBS), 2 mM of L-glutamine, 0.1 mM of non-essential amino acid, 1% of nucleoside mix, 0.1 mM of ß-mercaptoethanol, 1000U/ml of recombinant leukemia inhibitory factor and 50 U/ml of Penicillin/Streptomycin.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs were extracted using RNeasy plus mini kit (Qiagen)
|
| Label |
Cy3
|
| Label protocol |
cDNAs were generated using qScript (Qunta) and then labeled with Cy3
|
| |
|
| Hybridization protocol |
A hybridization mixture including the fragmented DNA and probe array controls were prepared, and then hybridized to the probe array during a 16-hour incubation
|
| Scan protocol |
GeneChips were scanned by Scanner 3000, Affymetrix
|
| Description |
Gene expression data after differentiation day4
|
| Data processing |
Robust multi-array (RMA) normalization method was applied to calculate averaged expression values from replicates
|
| |
|
| Submission date |
Jan 05, 2016 |
| Last update date |
Oct 01, 2016 |
| Contact name |
Bum-Kyu Lee |
| E-mail(s) |
bklee92@gmail.com
|
| Phone |
512-232-8046
|
| Organization name |
University of Texas at Austin
|
| Street address |
2506 Speedway Stop A5000
|
| City |
Austin |
| State/province |
TX |
| ZIP/Postal code |
78712 |
| Country |
USA |
| |
|
| Platform ID |
GPL8321 |
| Series (2) |
| GSE76558 |
Fbxl19 recruits Rnf20 to CpG Islands and promotes H2B mono-ubiquitination [Affymetrix] |
| GSE76570 |
Fbxl19 recruits Rnf20 to CpG Islands and promotes H2B mono-ubiquitination |
|
