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Sample GSM2033393 Query DataSets for GSM2033393
Status Public on May 31, 2016
Title TMK3
Sample type RNA
Source name Tympanic membrane keratinocyte
Organism Homo sapiens
Characteristics tissue: tympanic membrane
cell type: keratinocyte
Treatment protocol No treatment
Extracted molecule total RNA
Extraction protocol Total RNA from samples of cultivated TMK and EK cells was extracted using the miRNeasy mini kit from Qiagen
Label biotin
Label protocol Total RNA (300 ng) from each sample was prepared using the Genisphere FlashTag HSR kit(Genisphere, Hatfield, PA) according to manufacturer’s instructions.
Hybridization protocol Labeled sense strand DNA was hybridized to the GeneChip miRNA 3.0 Array (Affymetrix, Santa Clara, CA). The arrays were washed and stained using FS-450 fluidics station (Affymetrix).
Scan protocol Fluorescent images were scanned by Hewlett Packard Gene Array Scanner 3000 7G (Hewlett Packard, Palo Alto, CA).The scanned images were then analyzed using Affymetrix GeneChip Command Console
Description Primary cells from surgical patients
Data processing The CEL files were imported into Partek Genomics Suite software (Partek, Inc., St. Louis, MO). Robust microarray analysis (RMA) was applied for normalization.
Submission date Jan 08, 2016
Last update date May 31, 2016
Contact name Peder Aabel
Organization name Akershus University Hospital
Department ENT
Street address Sykehusvn. 27
City Lorenskog
ZIP/Postal code 1478
Country Norway
Platform ID GPL16384
Series (2)
GSE76673 Expression data of human tympanic membrane cells and epidermal keratinocytes [miRNA]
GSE76674 Expression data of human tympanic membrane cells and epidermal keratinocytes

Data table header descriptions
VALUE RMA signal

Data table
14q0_st 1.16756
14qI-1_st 0.901371
14qI-1_x_st 1.15946
14qI-2_st 1.23459
14qI-3_x_st 0.921693
14qI-4_st 0.922963
14qI-4_x_st 1.08819
14qI-5_st 1.03352
14qI-6_st 1.24664
14qI-6_x_st 1.21801
14qI-7_st 1.06297
14qI-8_st 1.09346
14qI-8_x_st 0.832419
14qI-9_x_st 0.870182
14qII-10_st 0.991524
14qII-10_x_st 1.07079
14qII-11_st 1.11528
14qII-11_x_st 1.16556
14qII-12_st 0.718935
14qII-12_x_st 1.80037

Total number of rows: 25119

Table truncated, full table size 622 Kbytes.

Supplementary file Size Download File type/resource
GSM2033393__52090200931520121714418819660468.CEL.gz 807.5 Kb (ftp)(http) CEL
Processed data included within Sample table

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