|
| Status |
Public on May 31, 2016 |
| Title |
TMK3 |
| Sample type |
RNA |
| |
|
| Source name |
Tympanic membrane keratinocyte
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: tympanic membrane
cell type: keratinocyte
|
| Treatment protocol |
No treatment
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from samples of cultivated TMK and EK cells was extracted using the miRNeasy mini kit from Qiagen
|
| Label |
biotin
|
| Label protocol |
Total RNA (300 ng) from each sample was prepared using the Genisphere FlashTag HSR kit(Genisphere, Hatfield, PA) according to manufacturer’s instructions.
|
| |
|
| Hybridization protocol |
Labeled sense strand DNA was hybridized to the GeneChip miRNA 3.0 Array (Affymetrix, Santa Clara, CA). The arrays were washed and stained using FS-450 fluidics station (Affymetrix).
|
| Scan protocol |
Fluorescent images were scanned by Hewlett Packard Gene Array Scanner 3000 7G (Hewlett Packard, Palo Alto, CA).The scanned images were then analyzed using Affymetrix GeneChip Command Console
|
| Description |
Primary cells from surgical patients
|
| Data processing |
The CEL files were imported into Partek Genomics Suite software (Partek, Inc., St. Louis, MO). Robust microarray analysis (RMA) was applied for normalization.
miRNA-3_0-st-v3.pgf
miRNA-3_0.annotations.psname.20121203.probeset.csv
|
| |
|
| Submission date |
Jan 08, 2016 |
| Last update date |
May 31, 2016 |
| Contact name |
Peder Aabel |
| E-mail(s) |
peder.aabel@medisin.uio.no
|
| Organization name |
Akershus University Hospital
|
| Department |
ENT
|
| Street address |
Sykehusvn. 27
|
| City |
Lorenskog |
| ZIP/Postal code |
1478 |
| Country |
Norway |
| |
|
| Platform ID |
GPL16384 |
| Series (2) |
| GSE76673 |
Expression data of human tympanic membrane cells and epidermal keratinocytes [miRNA] |
| GSE76674 |
Expression data of human tympanic membrane cells and epidermal keratinocytes |
|
