|
| Status |
Public on May 19, 2016 |
| Title |
Control shRNA replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
Control shRNA
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
shRNA: FLUC
genotype/variation: control
tissue: Large intestine
replicate: 2
|
| Treatment protocol |
Induction of shRNA expression was performed in 4-week-old shRNA/rtTA mice by supplying doxycycline (DOX) at 1 mg/ml in the drinking water (plus 5% sucrose) for 14 days. The DOX-supplemented water was changed every 4 days.
|
| Growth protocol |
All mice strains were maintained on a C57BL/6 background.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from the large intestine of DOX-treated (14 days) FLuc.1309/rtTA, DHX9.837/rtTA, and DHX9.1271/rtTA mice using TRIzol as per the manufacturer’s instructions (Life Technologies).
|
| Label |
biotin
|
| Label protocol |
The Affymetrix kit for Affymetrix Mouse Gene 2.0 ST DNA micro arrays using conditions recommended by the manufacturer.
|
| |
|
| Hybridization protocol |
The resulting labeled samples were hybridized to the Affymetrix Mouse Gene 2.0 ST Array from Affymetrix according the instructions of the manufacturer
|
| Scan protocol |
Arrays were scanned using the GeneArray Scanner 3000 according to the instructions from the manufacturer.
|
| Description |
Gene expression data from control
|
| Data processing |
Data were summarized and normalized using the oligo package version 1.30.0 by applying robust multiarray average (rma) in R version 3.1.1 (www.r-project.org) and bioconductor version 3.0
|
| |
|
| Submission date |
Jan 12, 2016 |
| Last update date |
May 19, 2016 |
| Contact name |
Ola Larsson |
| E-mail(s) |
ola.larsson@ki.se
|
| Phone |
+46 (0)8 517 73280
|
| Organization name |
Karolinska Institutet
|
| Department |
Department of oncology-pathology
|
| Street address |
CCK, R8:01
|
| City |
Stockholm |
| ZIP/Postal code |
171 76 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL20710 |
| Series (1) |
| GSE76765 |
Tumor Cell Survival Dependence on the DExH-Box Helicase DHX9 |
|
