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Status |
Public on May 19, 2016 |
Title |
DHX9-837 shRNA replicate 3 |
Sample type |
RNA |
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Source name |
DHX9-837 shRNA
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Organism |
Mus musculus |
Characteristics |
strain: C57BL/6 shRNA: DHX9_837 genotype/variation: DHX9 knockdown tissue: Large intestine replicate: 3
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Treatment protocol |
Induction of shRNA expression was performed in 4-week-old shRNA/rtTA mice by supplying doxycycline (DOX) at 1 mg/ml in the drinking water (plus 5% sucrose) for 14 days. The DOX-supplemented water was changed every 4 days.
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Growth protocol |
All mice strains were maintained on a C57BL/6 background.
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Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated from the large intestine of DOX-treated (14 days) FLuc.1309/rtTA, DHX9.837/rtTA, and DHX9.1271/rtTA mice using TRIzol as per the manufacturer’s instructions (Life Technologies).
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Label |
biotin
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Label protocol |
The Affymetrix kit for Affymetrix Mouse Gene 2.0 ST DNA micro arrays using conditions recommended by the manufacturer.
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Hybridization protocol |
The resulting labeled samples were hybridized to the Affymetrix Mouse Gene 2.0 ST Array from Affymetrix according the instructions of the manufacturer
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Scan protocol |
Arrays were scanned using the GeneArray Scanner 3000 according to the instructions from the manufacturer.
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Description |
Gene expression data from DHX9-shRNA837
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Data processing |
Data were summarized and normalized using the oligo package version 1.30.0 by applying robust multiarray average (rma) in R version 3.1.1 (www.r-project.org) and bioconductor version 3.0
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Submission date |
Jan 12, 2016 |
Last update date |
May 19, 2016 |
Contact name |
Ola Larsson |
E-mail(s) |
ola.larsson@ki.se
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Phone |
+46 (0)8 517 73280
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Organization name |
Karolinska Institutet
|
Department |
Department of oncology-pathology
|
Street address |
CCK, R8:01
|
City |
Stockholm |
ZIP/Postal code |
171 76 |
Country |
Sweden |
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|
Platform ID |
GPL20710 |
Series (1) |
GSE76765 |
Tumor Cell Survival Dependence on the DExH-Box Helicase DHX9 |
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