|
| Status |
Public on Jul 24, 2016 |
| Title |
ANXA2_3 |
| Sample type |
RNA |
| |
|
| Source name |
Primary Glioblastoma Cells
|
| Organism |
Homo sapiens |
| Characteristics |
antibody: isotype control
cell type: primary glioblastoma cells
|
| Treatment protocol |
GBM cells were treated with an anti-ANXA2 monoclonal antibody or an Isotype control antibody at a final concentration of 20µg/ml and then cultured for 48 hours before total RNA extraction.
|
| Growth protocol |
GBM cells were cultured on fibronectin-coated dishes in DMEM/F12 medium supplemented with BIT9500, 20ng/ml basic Fibroblast Growth Factor and 20ng/ml Epidermal Growth Factor in an atmosphere of 2% oxygen, 5% carbon dioxide and balanced nitrogen in a Ruskinn C300 system for hypoxic cell culture.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from cultured cells treated with an isotype control or an ANXA2 neutralizing antibody using Trizol in 4 independent experiments
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100ng of Total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 11 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Scan GCS3000.
|
| Description |
Gene expression data from primary GBM cells treated with an isotype control antibody
|
| Data processing |
The data were normalized using R software [www.r-ptoject.org] with BioConductor package [www.bioconductor.org] using RMA
|
| |
|
| Submission date |
Jan 12, 2016 |
| Last update date |
Jul 24, 2016 |
| Contact name |
silvia bresolin |
| E-mail(s) |
silvia.bresolin@unipd.it
|
| Phone |
+390498215487
|
| Organization name |
università di padova
|
| Department |
department of women's and children's health
|
| Lab |
SSD ematologia-clinica sperimentale
|
| Street address |
via giustiniani 3
|
| City |
padova |
| State/province |
padova |
| ZIP/Postal code |
35128 |
| Country |
Italy |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE76786 |
Annexin 2A sustains glioblastoma cell dissemination and proliferation affecting patient prognosis |
|
