|
| Status |
Public on Jan 13, 2016 |
| Title |
control root 1 |
| Sample type |
RNA |
| |
|
| Source name |
Root tissue, untreated with PEG6000
|
| Organism |
Leucaena leucocephala |
| Characteristics |
developmental stage: seedling
tissue: root
treatment: seedlings watered with Hoagland's solution
stress: control
|
| Treatment protocol |
Fifteen % PEG6000 in Hoagland's solution was used as a drought treatment. After 48 hours, shoots and roots of the seedlings were collected and immediately frozen in liquid nitrogen. Untreated seedlings, collected at the same time, were used as control.
|
| Growth protocol |
Leucaena leucocephala seedlings were grown in pots containing a vermiculite-perlite mixture for four weeks at 25 °C +/- 2° C with a 16/8-h light/dark photoperiod with an irradiance of 30 µmol s-1 m-1. Hoagland's solution was used to water the seedlings.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen Rneasy Plant Kit and Takara Fruit-mate were used to extract total RNA from the tissues of L. leucocephala.
|
| Label |
Cy3
|
| Label protocol |
Labelling was performed by Carver Center for Genomics, University of Iowa, IA, USA following Agilent standard operating protocol.
|
| |
|
| Hybridization protocol |
Hybridization was performed by Carver Center for Genomics, University of Iowa, IA, USA following Agilent standard operating protocol.
|
| Scan protocol |
Scanning was performed by Carver Center for Genomics, University of Iowa, IA, USA following Agilent standard operating protocol.
|
| Description |
CR1
|
| Data processing |
All the data extraction was done using the default setting for one-color gene expression arrays (protocol GE1_1105_Oct12) using the Feature Extraction v.11.5.1.1 software
[final, processed data] average intensity for target
|
| |
|
| Submission date |
Jan 13, 2016 |
| Last update date |
Jan 13, 2016 |
| Contact name |
Kazue Ishihara |
| E-mail(s) |
kazuei@hawaii.edu
|
| Organization name |
University of Hawaii at Manoa
|
| Department |
Molecular Biosciences and Bioengineering
|
| Street address |
1955 East-West Rd AgSci 218
|
| City |
Honolulu |
| State/province |
HI |
| ZIP/Postal code |
96822 |
| Country |
USA |
| |
|
| Platform ID |
GPL21327 |
| Series (1) |
| GSE76810 |
Determination of genes expressed in response to drought in root and shoot of Leucaena leucocephala |
|
