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Sample GSM2046322 Query DataSets for GSM2046322
Status Public on Jan 27, 2016
Title Pollen Control M82, rep3
Sample type RNA
 
Source name Anthers one day preanthesis
Organism Solanum lycopersicum
Characteristics tissue: Anthers (pollen)
line: parental
Treatment protocol All plants were treated equally. Collected tissue was frozen in liquid nitrogen.
Growth protocol Standard greenhouse cultivation. Three-week seedlings were transferred to individual 3.75L pots, grown in climate-controlled facility. Watered daily, feritlized weekly
Extracted molecule total RNA
Extraction protocol Trizol, RNAeasy purification (Qiagen) following manufacturer's instructions
Label biotin
Label protocol Single cycle
 
Hybridization protocol Standard
Scan protocol Affy GCOS
Description Gene expression data from anthers from domesticated tomato (control)
Data processing CEL files were processed using the rma() function from the Affy package in R, with default settings.
 
Submission date Jan 26, 2016
Last update date Jan 27, 2016
Contact name Rafael F Guerrero
Organization name Indiana University
Department Biology
Street address 1001 E 3rd St
City Bloomington
State/province IN - Indiana
ZIP/Postal code 47405
Country USA
 
Platform ID GPL4741
Series (1)
GSE77232 Pre-anthesis anthers of S. pennellii introgression lines

Data table header descriptions
ID_REF
VALUE log2(intensity)

Data table
ID_REF VALUE
AFFX-BioB-3_at 7.513843607
AFFX-BioB-5_at 7.280601589
AFFX-BioB-M_at 8.07005785
AFFX-BioC-3_at 9.209910499
AFFX-BioC-5_at 8.923805125
AFFX-BioDn-3_at 10.91585546
AFFX-BioDn-5_at 10.29329806
AFFX-CreX-3_at 12.60437694
AFFX-CreX-5_at 12.26428467
AFFX-DapX-3_at 9.408643929
AFFX-DapX-5_at 7.442459968
AFFX-DapX-M_at 8.913881396
AFFX-Le_17SrRNA_at 6.841086339
AFFX-Le_25SrRNA_at 8.427565419
AFFX-Le_GlutTrans_3_at 12.75777971
AFFX-Le_GlutTrans_5_at 12.38470315
AFFX-Le_GlutTrans_M_r_at 11.7365446
AFFX-Le_PhyB2_3_at 6.897082084
AFFX-Le_PhyB2_5_at 4.210220208
AFFX-Le_PhyB2_M_at 3.964031253

Total number of rows: 10209

Table truncated, full table size 307 Kbytes.




Supplementary file Size Download File type/resource
GSM2046322_LM01T003.CEL.gz 1.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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