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Sample GSM2046324 Query DataSets for GSM2046324
Status Public on Jan 27, 2016
Title Pollen Control M82, rep5
Sample type RNA
 
Source name Anthers one day preanthesis
Organism Solanum lycopersicum
Characteristics tissue: Anthers (pollen)
line: parental
Treatment protocol All plants were treated equally. Collected tissue was frozen in liquid nitrogen.
Growth protocol Standard greenhouse cultivation. Three-week seedlings were transferred to individual 3.75L pots, grown in climate-controlled facility. Watered daily, feritlized weekly
Extracted molecule total RNA
Extraction protocol Trizol, RNAeasy purification (Qiagen) following manufacturer's instructions
Label biotin
Label protocol Single cycle
 
Hybridization protocol Standard
Scan protocol Affy GCOS
Description Gene expression data from anthers from domesticated tomato (control)
Data processing CEL files were processed using the rma() function from the Affy package in R, with default settings.
 
Submission date Jan 26, 2016
Last update date Jan 27, 2016
Contact name Rafael F Guerrero
Organization name Indiana University
Department Biology
Street address 1001 E 3rd St
City Bloomington
State/province IN - Indiana
ZIP/Postal code 47405
Country USA
 
Platform ID GPL4741
Series (1)
GSE77232 Pre-anthesis anthers of S. pennellii introgression lines

Data table header descriptions
ID_REF
VALUE log2(intensity)

Data table
ID_REF VALUE
AFFX-BioB-3_at 7.199777913
AFFX-BioB-5_at 7.064183509
AFFX-BioB-M_at 7.776631247
AFFX-BioC-3_at 8.944103625
AFFX-BioC-5_at 8.750790963
AFFX-BioDn-3_at 10.72933714
AFFX-BioDn-5_at 10.05097452
AFFX-CreX-3_at 12.5228588
AFFX-CreX-5_at 12.12867662
AFFX-DapX-3_at 9.220755915
AFFX-DapX-5_at 7.250600309
AFFX-DapX-M_at 8.636860971
AFFX-Le_17SrRNA_at 6.98266954
AFFX-Le_25SrRNA_at 8.242951529
AFFX-Le_GlutTrans_3_at 11.50741425
AFFX-Le_GlutTrans_5_at 11.20469742
AFFX-Le_GlutTrans_M_r_at 10.39115268
AFFX-Le_PhyB2_3_at 6.93093926
AFFX-Le_PhyB2_5_at 4.305783271
AFFX-Le_PhyB2_M_at 3.801650736

Total number of rows: 10209

Table truncated, full table size 307 Kbytes.




Supplementary file Size Download File type/resource
GSM2046324_LM01T005.CEL.gz 1.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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