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Sample GSM2046325 Query DataSets for GSM2046325
Status Public on Jan 27, 2016
Title Pollen Control M82, rep6
Sample type RNA
 
Source name Anthers one day preanthesis
Organism Solanum lycopersicum
Characteristics tissue: Anthers (pollen)
line: parental
Treatment protocol All plants were treated equally. Collected tissue was frozen in liquid nitrogen.
Growth protocol Standard greenhouse cultivation. Three-week seedlings were transferred to individual 3.75L pots, grown in climate-controlled facility. Watered daily, feritlized weekly
Extracted molecule total RNA
Extraction protocol Trizol, RNAeasy purification (Qiagen) following manufacturer's instructions
Label biotin
Label protocol Single cycle
 
Hybridization protocol Standard
Scan protocol Affy GCOS
Description Gene expression data from anthers from domesticated tomato (control)
Data processing CEL files were processed using the rma() function from the Affy package in R, with default settings.
 
Submission date Jan 26, 2016
Last update date Jan 27, 2016
Contact name Rafael F Guerrero
Organization name Indiana University
Department Biology
Street address 1001 E 3rd St
City Bloomington
State/province IN - Indiana
ZIP/Postal code 47405
Country USA
 
Platform ID GPL4741
Series (1)
GSE77232 Pre-anthesis anthers of S. pennellii introgression lines

Data table header descriptions
ID_REF
VALUE log2(intensity)

Data table
ID_REF VALUE
AFFX-BioB-3_at 7.130321171
AFFX-BioB-5_at 7.04831101
AFFX-BioB-M_at 7.850547044
AFFX-BioC-3_at 9.087082719
AFFX-BioC-5_at 8.727469877
AFFX-BioDn-3_at 10.76173203
AFFX-BioDn-5_at 10.08736276
AFFX-CreX-3_at 12.55331525
AFFX-CreX-5_at 12.16013521
AFFX-DapX-3_at 9.267442745
AFFX-DapX-5_at 7.197740013
AFFX-DapX-M_at 8.767854581
AFFX-Le_17SrRNA_at 6.574184955
AFFX-Le_25SrRNA_at 8.180918799
AFFX-Le_GlutTrans_3_at 11.18294271
AFFX-Le_GlutTrans_5_at 10.90113706
AFFX-Le_GlutTrans_M_r_at 10.16038245
AFFX-Le_PhyB2_3_at 7.135915285
AFFX-Le_PhyB2_5_at 4.418133006
AFFX-Le_PhyB2_M_at 3.848948827

Total number of rows: 10209

Table truncated, full table size 307 Kbytes.




Supplementary file Size Download File type/resource
GSM2046325_LM01T006.CEL.gz 1.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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