|
| Status |
Public on Jan 27, 2016 |
| Title |
IL-4_2, rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Anthers one day preanthesis
|
| Organism |
Solanum lycopersicum x Solanum pennellii |
| Characteristics |
tissue: Anthers (pollen)
line: IL-4_2
|
| Treatment protocol |
All plants were treated equally. Collected tissue was frozen in liquid nitrogen.
|
| Growth protocol |
Standard greenhouse cultivation. Three-week seedlings were transferred to individual 3.75L pots, grown in climate-controlled facility. Watered daily, feritlized weekly
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol, RNAeasy purification (Qiagen) following manufacturer's instructions
|
| Label |
biotin
|
| Label protocol |
Single cycle
|
| |
|
| Hybridization protocol |
Standard
|
| Scan protocol |
Affy GCOS
|
| Description |
Gene expression data from anthers from introgression line IL-4_2
|
| Data processing |
CEL files were processed using the rma() function from the Affy package in R, with default settings.
|
| |
|
| Submission date |
Jan 26, 2016 |
| Last update date |
Jan 27, 2016 |
| Contact name |
Rafael F Guerrero |
| Organization name |
Indiana University
|
| Department |
Biology
|
| Street address |
1001 E 3rd St
|
| City |
Bloomington |
| State/province |
IN - Indiana |
| ZIP/Postal code |
47405 |
| Country |
USA |
| |
|
| Platform ID |
GPL4741 |
| Series (1) |
| GSE77232 |
Pre-anthesis anthers of S. pennellii introgression lines |
|
