|
| Status |
Public on Sep 26, 2017 |
| Title |
Pancreas from a pancreas-specific Jarid2 knockout (KO) E15.5 mouse embryo 2 |
| Sample type |
RNA |
| |
|
| Source name |
Pancreas from a pancreas-specific Jarid2 knockout (KO) E15.5 mouse embryo
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
tissue: Embryonic pancreas
genotype: Jarid2 knock-out
age: E15.5
|
| Growth protocol |
Pancreases from pancreas-specific Jarid2 knockout and control embryos were harvested at embryonic day 15.5.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with the Rneasy mini kit from Qiagen
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol through two cycles of cDNA synthesis (50 ng starting total RNA).
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA was hybridized to Genechip Mouse 430 2.0 Affymetrix arrays, washed and stained according to the standard Affymetrix protocol.
|
| Scan protocol |
GeneChips were scanned according to the standard Affymetrix protocol (Affymetrix Genechip Command Console program 3.0.0.1214, scanner GCS 3000-7G)
|
| Description |
Gene expression data from old islets at 11mM glucose
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
|
| |
|
| Submission date |
Jan 26, 2016 |
| Last update date |
Sep 27, 2017 |
| Contact name |
Joan Marc Servitja |
| E-mail(s) |
servitja@clinic.ub.es
|
| Organization name |
IDIBAPS
|
| Street address |
Rosselló, 153
|
| City |
Barcelona |
| State/province |
Catalonia |
| ZIP/Postal code |
08036 |
| Country |
Spain |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE77238 |
Expression profiling of E15.5 pancreases from pancreas-specific Jarid2 knockout (KO) embryos. |
|
