|
| Status |
Public on Jan 31, 2017 |
| Title |
Multiple myeloma. Pair 19. Diagnosis. [DNA copy number] |
| Sample type |
genomic |
| |
|
| Source name |
MM patient_diagnosis
|
| Organism |
Homo sapiens |
| Characteristics |
subject condition: Multiple myeloma (MM) patient
sample pair id: pair 19
gender: Male
age: 65
patient status: diagnosis
tissue: Bone marrow
cell type: CD 138+ plasma cells
|
| Treatment protocol |
Not applicable
|
| Growth protocol |
Not applicable
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Tumors were extracted and cells were collected. Total RNA from disgregated cells was extracted using Buffer RLT plus (Qiagen, CA, USA) and purified with All prep DNA/RNA mini Kit (Qiagen, CA, USA).
|
| Label |
biotin
|
| Label protocol |
Labelling and hybridizations were performed according to protocols from Affymetrix
|
| |
|
| Hybridization protocol |
DNA was restriction digested, PCR amplified, fragmented, labeled and hybridized to each array according to the manufacturer's instructions.
|
| Scan protocol |
The Arrays were then washed using Affymetrix fluidics stations, and scanned using the Gene Chip Scanner 3000.
|
| Description |
P19D_2577
|
| Data processing |
ChAS console from Affymetrix was used to assess genome wide copy number frequencies
The normalized data matrix contains Weighted Log2 Ratios
The *cychp files were generated as following:
affymetrix-algorithm-name = CYTO2
affymetrix-algorithm-version = 1.16.0
program-name = apt-copynumber-cyto.exe
program-version = 1.16.0
|
| |
|
| Submission date |
Feb 03, 2016 |
| Last update date |
Jan 31, 2017 |
| Contact name |
Norma C. Gutiérrez |
| E-mail(s) |
normagu@usal.es
|
| Phone |
+34923291384
|
| Organization name |
Centro de Investigación del Cáncer de Salamanca
|
| Department |
Hematologia
|
| Lab |
12
|
| Street address |
Campus Miguel de Unamuno
|
| City |
Salamanca |
| State/province |
Salamanca |
| ZIP/Postal code |
37007 |
| Country |
Spain |
| |
|
| Platform ID |
GPL18637 |
| Series (2) |
| GSE77536 |
Integrative analysis of DNA copy number, DNA methylation and gene expression in multiple myeloma reveals alterations related to relapse [DNA copy number] |
| GSE77540 |
Integrative analysis of DNA copy number, DNA methylation and gene expression in multiple myeloma reveals alterations related to relapse |
|
