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Status |
Public on Feb 22, 2017 |
Title |
FACS-sorted human ICC_replicate_1 |
Sample type |
RNA |
|
|
Source name |
FACS-sorted human ICC
|
Organism |
Homo sapiens |
Characteristics |
tissue source: De-identified human gastric tissues cell type: FACS-sorted human intersitial cells of Cajal (ICC)
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was prepared from cells using the Total RNA Purification Kit (Norgen, Ontario, Canada). RNA quality was assessed by Agilent Bioanalyzer (Santa Clara, CA).
|
Label |
biotin/streptavidin-phycoerythrin
|
Label protocol |
The Ovation Pico WTA system (NuGEN, San Carlos, CA) was used to prepare cDNA by single primer isothermal amplification (SPIA®).
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Hybridization protocol |
The SPIA cDNA was then bead-purified, fragmented, and hybridized onto Affymetrix GeneChip Human Genome U133 Plus 2.0 array (Affymetrix, Santa Clara, CA). Subsequent to hybridization, the arrays were washed and stained with streptavidin-phycoerythrin.
|
Scan protocol |
The arrays were scanned in an Affymetrix GeneChip® Scanner 3000 (Santa Clara, CA). All control parameters were confirmed to be within normal ranges before normalization and data reduction was initiated.
|
Description |
ICC_1
|
Data processing |
Analysis was performed using Bioconductor, a publicly available group of software packages. Affy was used to preprocess individual probe intensities from CEL files into RMA-normalized values.
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|
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Submission date |
Feb 11, 2016 |
Last update date |
Feb 22, 2017 |
Contact name |
Tamas Ordog |
E-mail(s) |
ordog.tamas@mayo.edu
|
Organization name |
Mayo Clinic
|
Street address |
200 1st St SW
|
City |
Rochester |
State/province |
MN |
ZIP/Postal code |
55905 |
Country |
USA |
|
|
Platform ID |
GPL570 |
Series (1) |
GSE77839 |
Transcriptome analysis of a FACS-sorted human intersitial cells of Cajal (ICC) [array] |
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