|
| Status |
Public on Oct 15, 2022 |
| Title |
lncRNA profile of 158HCC |
| Sample type |
RNA |
| |
|
| Source name |
158HCC
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: serum
disease state: hepatocellular carcinoma
data set: validation
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from 1.5 - 2.0 mL serum using Plasma/Serum Circulating and Exosomal RNA Purification Mini Kit (Cat # 51000) according to manufacturer’s instructions (Norgen Biotek, Canada).
|
| Label |
Cyanine-5
|
| Label protocol |
In labeling reaction, 400 - 600 ng of total RNAs were used for reverse transcription using GoScript™ Reverse Transcription system (Promega, USA) with random primers and Cyanine-5-dUTP (Cat # ENZ-42502, Enzo Life Sciences, USA). Labeled RNAs were purified using Micro Bio-Spin 30 column (Bio-Rad, USA).
|
| |
|
| Hybridization protocol |
The labeled RNAs were hybridized to the array with a 1×hybridization solution (5× Denhart’s solution, 0.5% SDS, 5× SSC) in a Hybridization Chamber (Corning Inc, Corning, NY, USA) at 45°C for 12 to 18 hours.
|
| Scan protocol |
Scanned in a LuxScanTM -10K Scanner (capitalBio Inc.,Beijing, China).
|
| Data processing |
Firstly, we gridded the microarray image using GenPix Pro 6.0 program, and subtracted background and normalized the data (quantile normalization) using GPR analysis software(edited in-house). Then we computed the average intensity of the duplicated probes and transformed them into log2 value.
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| |
|
| Submission date |
Feb 22, 2016 |
| Last update date |
Oct 15, 2022 |
| Contact name |
Li-Si Zeng |
| E-mail(s) |
sisi713@163.com
|
| Phone |
13016064632
|
| Organization name |
Sun Yat-Sen University
|
| Department |
Sun Yat-Sen University Cancer Center
|
| Lab |
State Key Laboratory of Oncology in South China
|
| Street address |
651 Dongfeng Road East
|
| City |
Guangzhou |
| State/province |
Guangdong |
| ZIP/Postal code |
510060 |
| Country |
China |
| |
|
| Platform ID |
GPL21494 |
| Series (1) |
| GSE78160 |
Identification of a serum 48-lncRNA signature as diagnostic marker for hepatocellular carcinoma and liver cirrhosis |
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