|
| Status |
Public on Aug 25, 2017 |
| Title |
CUTLL Vorinostat rep 3 |
| Sample type |
RNA |
| |
|
| Source name |
CUTLL Cell Line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: CUTLL1
tissue: TALL Leukemia
treatment: Vorinostat 24h
|
| Treatment protocol |
CUTLL1 cells were treated with Withaferin A or DMSO for 24 hours.
|
| Growth protocol |
CUTLL1 cells were cultured in standard conditions in RPMI 1640 media supplemented with 10% FBS and 1% Penicillin/Streptomycin
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA isolation from cell lines was performed in 6 replicates using the RNeasy Micro kit (QIAGEN) according to manufacturer’s protocol.
|
| Label |
biotin
|
| Label protocol |
10 ng of RNA from each sample was amplified using the Ovation PicoSL WTA System (NuGen), labeled by the Encore BiotinIL Module (NuGen)
|
| |
|
| Hybridization protocol |
hybridized to the Illumina HumanT-12v4 Expression BeadChip Expression BeadChip according to manufacturer’s protocol
|
| Scan protocol |
Standard scan according to manufacturer's protocol
|
| Description |
replicate 3
|
| Data processing |
Raw gene expression data were log2 transformed and quantile-normalized using MATLAB.
|
| |
|
| Submission date |
Feb 23, 2016 |
| Last update date |
Aug 25, 2017 |
| Contact name |
Alberto Ambesi-Impiombato |
| Organization name |
Columbia University
|
| Department |
ICG
|
| Street address |
1130 St. Nicholas Avenue
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (2) |
| GSE78188 |
Gene expression profiling of CUTLL cell lines upon 17 drug treatments |
| GSE78189 |
Synergistic antileukemic therapies in NOTCH-induced T-ALL |
|
