|
| Status |
Public on Feb 24, 2016 |
| Title |
Stealth-D3 vs Stealth-CTR cells |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
1192A
|
| Organism |
Mus musculus |
| Characteristics |
cell line: BCC cell line G2N2C
transfection: Stealth-D3
|
| Treatment protocol |
BCC cells were transfected with D3-stealth and ctr-stealth oligos and harvested 48 hours following the transfection.
|
| Growth protocol |
BCC cells were cultured under low calcium conditions with 8% Ca2+-chelated fetal bovine serum and keratinocyte growth factor
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions. Next we purifed with the RNeasy MinElute Cleanup Kit.
|
| Label |
Cy3
|
| Label protocol |
miRCURY LNA (TM) microRNA Array Power Labeling Kit
|
| |
|
| Channel 2 |
| Source name |
1192B
|
| Organism |
Mus musculus |
| Characteristics |
cell line: BCC cell line G2N2C
transfection: Stealth-ctr
|
| Treatment protocol |
BCC cells were transfected with D3-stealth and ctr-stealth oligos and harvested 48 hours following the transfection.
|
| Growth protocol |
BCC cells were cultured under low calcium conditions with 8% Ca2+-chelated fetal bovine serum and keratinocyte growth factor
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions. Next we purifed with the RNeasy MinElute Cleanup Kit.
|
| Label |
Cy5
|
| Label protocol |
miRCURY LNA (TM) microRNA Array Power Labeling Kit
|
| |
|
| |
| Hybridization protocol |
miRCURY LNA (TM) microRNA Array microarray kit instruction manual v9.2
|
| Scan protocol |
Scanner=GenePix 4000B [92213]
|
| Description |
Each channel represents: transfected BCC cells, pool of 3 different experiments
Slide13768400 1192A cy3 vs 1192B cy5
1192A = Stealth-D3 transfected BCC cells, 1192B = Stealth-ctr transfected BCC cells
|
| Data processing |
Genepix software package was used to generate an intensity value for each probe by taking the median spot intensity and subtracting the median local background. Bad spots or areas of the array with obvious defects were manually flagged. Flagged spots were not included in subsequent analyses.
LOWESS normalized, background subtracted data obtained from log2 of processed Cy5 signal/processed Cy3 signal. Agilent software was used.
|
| |
|
| Submission date |
Feb 24, 2016 |
| Last update date |
Feb 24, 2016 |
| Contact name |
Monica Dentice |
| E-mail(s) |
monica.dentice@unina.it
|
| Phone |
0817463848
|
| Organization name |
University of Naples "Federico II"
|
| Department |
Department of Clinical Medicine and Surgery
|
| Street address |
Via S. Pansini 5
|
| City |
Naples |
| ZIP/Postal code |
80131 |
| Country |
Italy |
| |
|
| Platform ID |
GPL7724 |
| Series (1) |
| GSE78265 |
Murine BCC Cells: dio3 interfered vs control |
|
