|
| Status |
Public on Jan 01, 2008 |
| Title |
Medial substantia nigra Parkinson's disease case 02 - B chip |
| Sample type |
RNA |
| |
|
| Source name |
Post-mortem medial substantia nigra
|
| Organism |
Homo sapiens |
| Characteristics |
age: 83; gender: M
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the RNeasy Mini Kit (Qiagen) according to the manufacturer's protocol (http://www.qiagen.com). Tissue samples were mechanically disrupted using a rotary homogenizer, lysed in buffer containing guanidine isothiocyanate (GITC) and then further homogenized (Shredder tubes, Qiagen).
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45oC to a Human Genome 133 B array (HG_U133B; Affymetrix). The GeneChips was washed and stained in an Affymetrix Fluidics Station 400. Standard Affymetrix Protocols were used (Affymetrix GeneChip Expression Analysis Technical Manual, 2001).
|
| Scan protocol |
Standard Affymetrix protocol (Affymetrix GeneChip Expression Analysis Technical Manual, 2001).
|
| Description |
Gene expression data from medial substantia nigra (MNPD02B)
|
| Data processing |
CEL files were read using ArrayAssist software (version 4.1, Stratagene) and PathwayStudio 5 (Ariadne). Intensity values were derived applying the (GeneChip) Robust Multi Array (RMA) algorithm.
|
| |
|
| Submission date |
Jul 09, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Linda B Moran |
| Organization name |
Imperial College London
|
| Department |
Neuropathology
|
| Street address |
Fulham Palace Road
|
| City |
London |
| ZIP/Postal code |
W6 8RF |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL97 |
| Series (1) |
| GSE8397 |
Expression profiling of the Parkinsonian Brain |
|
