|
Status |
Public on Mar 22, 2016 |
Title |
Osteosarcoma P01 |
Sample type |
RNA |
|
|
Source name |
Right Distal Femur
|
Organism |
Homo sapiens |
Characteristics |
ageatdiagnosis: 12 gender: F relapse: Yes dead: Yes survival-days: 1463
|
Treatment protocol |
In a -20oC cryostat, 10 slices, each 10 μm thick frozen tissue were cut (total thickness 100 μm) and placed in 50 mL centrifuge tube containing pre-chill RNAlater-ICE solution (Ambion).
|
Growth protocol |
NA
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using the mirVana miR Isolation Kit (Ambion) according to manufacturer’s instructions
|
Label |
FAM
|
Label protocol |
miR expression was performed using the TaqMan Array Human MicroRNA Cards (Applied Biosystems), according to manufacturer's protocol
|
|
|
Hybridization protocol |
n/a
|
Scan protocol |
n/a
|
Data processing |
For each miR, the expression level was determined by the threshold cycle method (2^(-ΔCt)) of relative quantification (ΔCt = Ct of sample – average Ct of endogenous controls of sample). Quantile normalization was applied to compensate for the technical differences present among all the samples.
|
|
|
Submission date |
Mar 14, 2016 |
Last update date |
Jun 27, 2018 |
Contact name |
Yidong Chen |
E-mail(s) |
cheny8@uthscsa.edu
|
Phone |
2105629163
|
Organization name |
UT Health Science Center at San Antonio
|
Department |
Population Health Sciences
|
Street address |
8403 Floyd Curl Drive, MSC 7784
|
City |
San Antonio |
State/province |
Texas |
ZIP/Postal code |
78229 |
Country |
USA |
|
|
Platform ID |
GPL15497 |
Series (1) |
GSE79181 |
In silico analyses of survival-associated microRNA signatures in pediatric osteosarcoma |
|