|
| Status |
Public on Mar 22, 2016 |
| Title |
Osteosarcoma P06 |
| Sample type |
RNA |
| |
|
| Source name |
Left Femur
|
| Organism |
Homo sapiens |
| Characteristics |
ageatdiagnosis: 16
gender: F
relapse: Yes
dead: No
survival-days: 1947
|
| Treatment protocol |
In a -20oC cryostat, 10 slices, each 10 μm thick frozen tissue were cut (total thickness 100 μm) and placed in 50 mL centrifuge tube containing pre-chill RNAlater-ICE solution (Ambion).
|
| Growth protocol |
NA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the mirVana miR Isolation Kit (Ambion) according to manufacturer’s instructions
|
| Label |
FAM
|
| Label protocol |
miR expression was performed using the TaqMan Array Human MicroRNA Cards (Applied Biosystems), according to manufacturer's protocol
|
| |
|
| Hybridization protocol |
n/a
|
| Scan protocol |
n/a
|
| Data processing |
For each miR, the expression level was determined by the threshold cycle method (2^(-ΔCt)) of relative quantification (ΔCt = Ct of sample – average Ct of endogenous controls of sample). Quantile normalization was applied to compensate for the technical differences present among all the samples.
|
| |
|
| Submission date |
Mar 14, 2016 |
| Last update date |
Jun 27, 2018 |
| Contact name |
Yidong Chen |
| E-mail(s) |
cheny8@uthscsa.edu
|
| Phone |
2105629163
|
| Organization name |
UT Health Science Center at San Antonio
|
| Department |
Population Health Sciences
|
| Street address |
8403 Floyd Curl Drive, MSC 7784
|
| City |
San Antonio |
| State/province |
Texas |
| ZIP/Postal code |
78229 |
| Country |
USA |
| |
|
| Platform ID |
GPL15497 |
| Series (1) |
| GSE79181 |
In silico analyses of survival-associated microRNA signatures in pediatric osteosarcoma |
|
