|
| Status |
Public on Aug 25, 2016 |
| Title |
17_IAPP_5 |
| Sample type |
RNA |
| |
|
| Source name |
C4BP
|
| Organism |
Rattus norvegicus |
| Characteristics |
cell line: INS-1
cell type: beta-cell line
treated with: 300 ug/ml C4b-binding protein (C4BP)
|
| Treatment protocol |
The cells were then challenged by adding 300 ug/ml monomeric IAPP alone or together with C4BP (300 ug/ml). DMSO (1%) as well as C4BP (300 ug/ml) alone were used as controls.
|
| Growth protocol |
The INS-1 cells were grown as 5 separate clones for 10 passages before plating in a 12-well plate (Nunc) at 100.000 cells per well and grown in complete RPMI 1640 medium to 70% confluency for approximately 48 h.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extraction and purification was done according to Qiagen RNeasy kit protocol.
|
| Label |
biotin
|
| Label protocol |
Samples were enzymatically fragmented and biotinylated using the WT Terminal Labeling Kit (Affymetrix)
|
| |
|
| Hybridization protocol |
Samples were hybridized using Affymetrix hybridization kit materials • Heat cocktails at 99° for 5 minutes, then 42° for 5 minutes centrifuge at max speed for 1 minute (N.B. this deviates from Affy SOP). • Transfer 200μl of hyb solution to each array, then tape holes and parafilm. • Hybridize 16 hours at 45° at 60rpm• Fluidics washing is FS450_0001
|
| Scan protocol |
Affymetrix Gene ChIP Scanner 3000 7G
|
| Description |
17_IAPP_5
|
| Data processing |
Data were processed using Expression Console software. Probe intensities, gene level expression were summarized and then quantile normalized.
|
| |
|
| Submission date |
Mar 15, 2016 |
| Last update date |
Aug 26, 2016 |
| Contact name |
Petter Storm |
| E-mail(s) |
petter.storm@med.lu.se
|
| Phone |
+46-40 39 12 27
|
| Organization name |
Lund University
|
| Department |
Developmental and Regenerative Neurobiology
|
| Street address |
Sölvegatan 19, BMC A11
|
| City |
Lund |
| ZIP/Postal code |
22184 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL17117 |
| Series (1) |
| GSE79224 |
C4b-binding protein protects beta-cells from islet amyloid polypeptide induced cytotoxicity. |
|
