|
| Status |
Public on Feb 09, 2017 |
| Title |
SLE DCs Replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
monocyte-derived DCs
|
| Organism |
Homo sapiens |
| Characteristics |
diagnosis: systemic lupus erythematosus
tissue: PBMC
age: day7
cell type: dendritic cells
|
| Growth protocol |
PBMC were isolated and monocyte-derived dendritic cells were sorted by positive selection of CD14+ monocytes using magnetic beads. The cells were then cultured for 5-7 days supplemented with GM-CSF and IL-4. For DC maturation, 1μg/ml LPS was added to the medium at day 6.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
Total RNA was amplified using the Low Input Quick Amp WT Labeling kit, according to the manufacturer’s instructions (Agilent Technologies, Santa Clara, USA). 100 ng of total RNA was reverse-transcribed into double-stranded cDNA, which was translated into cRNA in the presence of Cy3-dCTP and amplified by T7 RNA polymerase.
|
| |
|
| Hybridization protocol |
Oligoarray control targets and hybridization buffer (Agilent In Situ Hybridization Kit Plus) were added, and samples were applied to microarrays enclosed in Agilent SureHyb-enabled hybridization chambers. After hybridization, slides were washed sequential
|
| Scan protocol |
Microarray images were acquired using an Agilent DNA microarray scanner.
|
| Data processing |
Agilent Feature Extraction Software (V10.7) was used for background subtraction and Quantile normalization.
|
| |
|
| Submission date |
Mar 15, 2016 |
| Last update date |
Feb 09, 2017 |
| Contact name |
Yilun Wang |
| E-mail(s) |
yolendawang@163.com
|
| Organization name |
Fudan University
|
| Street address |
No.12 Wulumuqi Zhong Road
|
| City |
Shanghai |
| ZIP/Postal code |
200040 |
| Country |
China |
| |
|
| Platform ID |
GPL18402 |
| Series (1) |
| GSE79240 |
microRNA profilling in dendritic cells of systemic lupus erythematosus |
|
