|
| Status |
Public on Mar 17, 2018 |
| Title |
rat fed low corn oil diet, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
ACI rat fed a low corn oil diet for 18 months, biological replicate 2
|
| Organism |
Rattus norvegicus |
| Characteristics |
tissue: dorsolateral prostate
strain: ACI
age: 18 month old
gender: male
|
| Treatment protocol |
dorsolateral prostates were resected at the end of the 18 month treatment and half was used for RNA extraction and hybridization to affymetrix microarrays and the other half was used for histochemical and immunohistochemical analysis
|
| Growth protocol |
male ACI rats were placed on either a high corn oil diet or a low corn oil diet for 18 months.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Genome U34 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from prostate of ACI rats fed low corn oil diet for 18 months
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
Mar 17, 2016 |
| Last update date |
Mar 17, 2018 |
| Contact name |
Niradiz Reyes |
| Organization name |
Universidad de Cartagena
|
| Street address |
Campus de Zaragocilla. Facultad de Medicina
|
| City |
Cartagena |
| ZIP/Postal code |
01 |
| Country |
Colombia |
| |
|
| Platform ID |
GPL85 |
| Series (1) |
| GSE79349 |
Expression data from dorsolateral prostates of ACI rats fed corn oil diets |
|
