|
Status |
Public on Mar 18, 2017 |
Title |
ApoE KO HSC, biological rep2 |
Sample type |
RNA |
|
|
Source name |
Hematopoietic stem cells from ApoE KO mice
|
Organism |
Mus musculus |
Characteristics |
tissue: bone marrow cell type: hematopoietic stem cells genotype: ApoE-/- age: 3 month old mice
|
Growth protocol |
hematopoietic stem cells were sorted by flow cytometry
|
Extracted molecule |
total RNA |
Extraction protocol |
total RNA were isolated by mirVana miRNA isolation kit according to manufacturer's instructions (Applied Biosystems).
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 20 ng total RNA
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|
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Hybridization protocol |
Following fragmentation, 20 ng of total RNA were hybridized for 16 hr at 45C. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
Scan protocol |
GeneChips were scanned by the Affymetrix Scanner 3000(Affymetrix).
|
Description |
microRNA expression in hematopoietic stem cells
|
Data processing |
The scanned images were analyzed using Expression Console Software (version 1.3.1 Affymetrix)
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|
|
Submission date |
Mar 17, 2016 |
Last update date |
Mar 18, 2017 |
Contact name |
Lyne Khair |
Organization name |
UMASS MEDICAL SCHOOL
|
Department |
SURGERY
|
Street address |
368 PLANTATION STREET
|
City |
WORCESTER |
State/province |
MA |
ZIP/Postal code |
01605 |
Country |
USA |
|
|
Platform ID |
GPL21572 |
Series (1) |
GSE79361 |
Hypercholesterolemia increases the incidence of colorectal cancer by miR-101c-dependent downregulation of Tet1 in Hematopoietic Stem Cells |
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