|
| Status |
Public on Jun 01, 2017 |
| Title |
2080 |
| Sample type |
protein |
| |
|
| Source name |
Peripheral Blood
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Serum
type: Serous Ovarian Cancer
array batch: GST1 - 4; FLAG
|
| Extracted molecule |
protein |
| Extraction protocol |
Peripheral blood samples were drawn from the antecubital vein and serum was prepared according to a standardized protocol before freezing serum aliquots at -80oC
|
| Label |
Serum samples are not labeled
|
| Label protocol |
No lable was performed on the serum sample
|
| |
|
| Hybridization protocol |
Plasma hybridization was performed on TECAN HS 4800 Pro. 1. Protein arrays were blocked with 5% milk PBST (0.2% Tween) for 1 hour at RT; 2. 150ul 1:50 diluted serum was applied to each slide with medium agitation 16 hours at 4oC; 3. Slides were incubated with 150ul 1:500 dilution of Dylight 649-linked anti-human secondary antibodies (Jackson Immunoresearch) in milk PBST.
|
| Scan protocol |
Slides were scanned by TECAN PowerScanner at 50% gain, 50% laser and 10µm resolution.
|
| Description |
The serum was applied on the protein array, secondary anti-human IgG antibody used for detection.
|
| Data processing |
1. The background signal estimated by the 25th percentile of the negative control; 2. Normalize the array by the median.
|
| |
|
| Submission date |
Mar 22, 2016 |
| Last update date |
Jun 01, 2017 |
| Contact name |
Benjamin A Katchman |
| Organization name |
Arizona State University
|
| Street address |
727 E. Tyler St.
|
| City |
Tempe |
| State/province |
AZ |
| ZIP/Postal code |
85287 |
| Country |
USA |
| |
|
| Platform ID |
GPL21621 |
| Series (2) |
| GSE79498 |
Autoanitbody Biomarkers for the Early Detection of Serous Ovarian Cancer [Discovery Screen] |
| GSE79517 |
Autoanitbody Biomarkers for the Early Detection of Serous Ovarian Cancer |
|
