|
| Status |
Public on Apr 01, 2016 |
| Title |
Ets1 deleted replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
ICOS+ ILC2 cells cultued 2 week on OP9-DL1
|
| Organism |
Mus musculus |
| Characteristics |
cell type: ILC2 cells
genotype: ETS1-deleted
strain: C57BL/6
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were directly sorted into buffer RLT and RNA prepared using RNeasy Micro (Qiagen) according to manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared according to the standard Affymetrix; Small Sample Labelling Protocol v.2, with the exception that the second round of in vitro transcription (IVT) was performed using Affymetrix GeneChip Expression 3 amplification kit.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 microg of cRNA was hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
Chips were scanned using an Affymetrix GeneChip Scanner 3000. Chip target intensity was arbitrarily scaled to 100 before rendering CHP files.
|
| Data processing |
Expression values were calculated by RMA express (http://rmaexpress.bmbolstad.com/) using background adjustment and quantile normalization.
|
| |
|
| Submission date |
Mar 30, 2016 |
| Last update date |
Apr 02, 2016 |
| Contact name |
Barbara L Kee |
| Organization name |
The University of Chicago
|
| Department |
Pathology
|
| Street address |
924 E57th st
|
| City |
Chicago |
| State/province |
Illinois |
| ZIP/Postal code |
60637 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE79742 |
Expression data of ILC2 cells from Ets1-deleted and littermate control mice |
|
