|
| Status |
Public on Jan 24, 2008 |
| Title |
Control 4 hr |
| Sample type |
RNA |
| |
|
| Source name |
Rat testis control 4 hr
|
| Organism |
Rattus rattus |
| Characteristics |
rat testis 60 days old
|
| Treatment protocol |
Experimental details are found at Tash et al, Gamendazole, an orally active indazole carboxylic acid male contraceptive agent, targets HSP-90, eEF1A, and stimulates IL-1 transcription in Sertoli cells, Biol. Reprod. (2007) under review
|
| Growth protocol |
Adult male Long-Evans rats (60 day old) administered single ip dose of 6mg/kg Gamendazole or 25 mg/kg lonidamine or vehicle control (buffered DMSO) - testes harvested at 0 4 12 and 24 hr
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TriZol, Phase Lock Gel-Heavy
|
| Label |
biotin
|
| Label protocol |
Biotin labeled cRNA targets were prepared from 6.2ug total RNA using Affymetrix's single-cycle eukaryotic target preparation procedure (Expression Analysis Technical Manual, 2001, Affymetrix). Reverse Transcription was performed using Superscipt II Choice System (Invitogen). Invitro-Transcription and labeling was conducted using the GeneChip Expression 3' Amplification Reagent Kit (Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15ug of biotin labeled cRNA was hybridized for 16hr 28min at 45C, 60rpm in an Affymetrix Hybridization Oven 640 to a Rat230 2.0 GeneChip expression array. The washing and antibody amplification staining procedure utilizing R phycoerthrin-streptavidin was performed with the Affymetrix Fluidics Station 450 using the EukGE-WS2v5_450 fluidics protocol.
|
| Scan protocol |
Single scans were performed using the Affymetrix GeneChip 3000 scanner.
|
| Description |
Gene expression data
|
| Data processing |
The data were analyzed with Gene Chip Operating Software (GCOS 1.1) using Affymetrix default analysis settings as normalization method. http://www.affymetrix.com/support/technical/manual/expression_manual.affx
|
| |
|
| Submission date |
Jul 16, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Joseph S Tash |
| E-mail(s) |
jtash@kumc.edu
|
| Phone |
913-588-7421
|
| Fax |
913-588-7180
|
| Organization name |
University of Kansas Medical Center
|
| Department |
Center for Male Contraceptive Research and Drug Development
|
| Street address |
3901 Rainbow Blvd.
|
| City |
Kansas City |
| State/province |
KS |
| ZIP/Postal code |
66160 |
| Country |
USA |
| |
|
| Platform ID |
GPL1355 |
| Series (1) |
| GSE8485 |
In vivo administration of Gamendazole and Lonidamine |
|
