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Sample GSM210552 Query DataSets for GSM210552
Status Public on Sep 22, 2008
Title NIL WKY 3
Sample type RNA
 
Source name Neurointermediate lobe
Organism Rattus norvegicus
Characteristics Strain: Wistar-Kyoto
Gender: Male
Age: 10-12 weeks
Tissue: Neurointermediate lobe of the Pituitary
Extracted molecule total RNA
Extraction protocol Tissue was dissected in an RNase free manner and stored in RNAlater (Ambion) at -20C for no more than 1 month. Homogenisation in QIAzol was conducted mechanically and the aqueous phase separated using a PLG column (Eppendorf). RNA was further purified using RNeasy minelute cleanup kit (Qiagen). RNA quality was confirmed using nanodrop and bioanylizer 2100 (Agilent).
Label MessageAmpII aRNA Amplification kit (Ambion)
Label protocol Protocol available at http://www.AMBION.com/catalog/CatNum.php?1753
 
Hybridization protocol Standard Affymetrix procedures
Scan protocol Standard Affymetrix procedures
Description Sample represents the Neurointermediate lobe of the Pituitary from 5 untreated Wistar-kyoto rats
Data processing GCOS version 1.2
 
Submission date Jul 16, 2007
Last update date Aug 14, 2011
Contact name DAVID MURPHY
Organization name University of Bristol
Department Medicine and Dentistry
Lab Laboratories for Integrative Neuroscience and Endocrinolgy
Street address Whitson Street
City Bristol
State/province AVON
ZIP/Postal code BS1 3NY
Country United Kingdom
 
Platform ID GPL1355
Series (1)
GSE8491 The Transcriptome of the rat hypothalamo-neurohypophyseal system is highly strain-dependant

Data table header descriptions
ID_REF
VALUE GCOS calculated signal intensity
ABS_CALL Indication of the transcript being present (P), absent (A), marginal (M)
DETECTION P-VALUE p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 102.947 P 0.000296708
AFFX-BioB-M_at 189.509 P 4.42873e-05
AFFX-BioB-3_at 107.234 P 4.42873e-05
AFFX-BioC-5_at 413.928 P 4.42873e-05
AFFX-BioC-3_at 340.789 P 5.16732e-05
AFFX-BioDn-5_at 675.828 P 5.16732e-05
AFFX-BioDn-3_at 1736.51 P 5.16732e-05
AFFX-CreX-5_at 3887.1 P 5.16732e-05
AFFX-CreX-3_at 4507.34 P 4.42873e-05
AFFX-DapX-5_at 0.962077 A 0.574038
AFFX-DapX-M_at 1.37209 A 0.737173
AFFX-DapX-3_at 1.15557 A 0.876428
AFFX-LysX-5_at 4.75037 A 0.250796
AFFX-LysX-M_at 1.24581 A 0.737191
AFFX-LysX-3_at 3.40744 A 0.262754
AFFX-PheX-5_at 0.615018 A 0.910522
AFFX-PheX-M_at 0.73555 A 0.83416
AFFX-PheX-3_at 4.98682 A 0.559354
AFFX-ThrX-5_at 3.61459 A 0.58862
AFFX-ThrX-M_at 7.03655 A 0.262827

Total number of rows: 31099

Table truncated, full table size 950 Kbytes.




Supplementary file Size Download File type/resource
GSM210552.CEL.gz 2.5 Mb (ftp)(http) CEL
GSM210552.CHP.gz 165.3 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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