|
| Status |
Public on Dec 31, 2007 |
| Title |
chicken intestine 28 |
| Sample type |
RNA |
| |
|
| Source name |
Day14 chick intestine
|
| Organism |
Gallus gallus |
| Characteristics |
gender: male
strain:B
tissue: whole intestine
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated for both microarray and real-time absolute quantification reverse transcriptase polymerase chain reaction (AQRT-PCR) studies. Tissue samples were ground in a mortar and pestle under liquid nitrogen. Total RNA from intestinal segments of six male birds at each time point were isolated using the RNeasy Mini Kit (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Enzo BioArray High Yield RNA Transcipt Labeling Kit
10ul purified cDNA
12ul DEPC H2O
4ul 10x Hy reaction Buffer (1x final conc.)
4ul 10x Biotin Labeled Ribonucleotides (1x final Conc.)
4ul 10x DTT (1x final conc.)
4ul 10x RNase Inhibitor mix (1x final Conc.)
2ul 20x T7 RNA polymerase (1x final Conc.)
- Incubate at 37C 5 hours mixing every 0.5 hour.
|
| |
|
| Hybridization protocol |
- GeneChip is prehybridized with 200ul 1x Hybridization buffer at 45C 10min at 60rpm
- 200ul denatured Hybridization cocktail is applied to GeneChip
- GeneChip is hybridized 16 hr at 45C and 60rpm in GeneChip Hybridization Oven 640.
|
| Scan protocol |
Arrays were scanned with an Affymetrix GeneChip Scanner 3000 at 570nm.
|
| Description |
N/A
|
| Data processing |
GC-RMA
|
| |
|
| Submission date |
Jul 16, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Huifeng Li |
| E-mail(s) |
lihuifengtom@yahoo.com.cn
|
| Phone |
1-540-231-3183
|
| Fax |
1-540-231-3010
|
| Organization name |
Virginia Tech
|
| Department |
Animal and Poultry Sciences
|
| Lab |
Biotechnology
|
| Street address |
3570 Litton Reaves Hall
|
| City |
Blacksburg |
| State/province |
VA |
| ZIP/Postal code |
24061-0306 |
| Country |
USA |
| |
|
| Platform ID |
GPL3213 |
| Series (1) |
| GSE8495 |
Expression profiling of the solute carrier gene family in the chicken intestine |
|
