|
| Status |
Public on Apr 20, 2017 |
| Title |
Scrb_HG_biological replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
C2C12 mouse cell line
|
| Organism |
Mus musculus |
| Characteristics |
cell line: C2C12
protocol: transduced with siScramble retrovirus and treated with 10 mM glucose for 12 h.
|
| Treatment protocol |
C2C12 myoblasts were transduced with retroviruses expressing shRNAs targeting control or mouse Baf60c, differentiated into myotubes, and treated with 1mM or 10 mM glucose for 12 h, followed by RNA extraction.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction of total RNA using Trizol method was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
According to standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
According to standard Affymetrix protocol.
|
| Scan protocol |
According to standard Affymetrix protocol.
|
| Description |
Gene expression data from C2C12 myotubes transduced with siScramble retrovirus and treated with 10 mM glucose for 12 h.
|
| Data processing |
Data was analyzed using the affy Bioconductor packages implemented in the R-statistical environment. RMA was used to fit log2 expression values.
|
| |
|
| Submission date |
Apr 05, 2016 |
| Last update date |
Apr 20, 2017 |
| Contact name |
Zhuoxian Meng |
| E-mail(s) |
zxmeng@umich.edu
|
| Phone |
7346153510
|
| Organization name |
University of Michigan
|
| Street address |
210 Washtenaw Ave
|
| City |
Ann Arbor |
| State/province |
Michigan |
| ZIP/Postal code |
48109 |
| Country |
USA |
| |
|
| Platform ID |
GPL17400 |
| Series (1) |
| GSE79925 |
Glucose sensing by skeletal myocytes couples nutrient signaling to systemic homeostasis |
|
