|
| Status |
Public on Sep 14, 2016 |
| Title |
A. lyrata salt, moderate conc., late time point rep 2 |
| Sample type |
RNA |
| |
|
| Source name |
shoot tissues, 250mM NaCl treatment, 27h post treatment harvest
|
| Organism |
Arabidopsis lyrata subsp. lyrata |
| Characteristics |
protocol: 250mM NaCl treatment
time point: 27h post treatment harvest
tissue: shoots freeze frozen
|
| Treatment protocol |
Tissues were harvested at 3h and 27h post NaCl treatment of moderate (250mM) and high (500 mM) concentrations.
|
| Growth protocol |
Six weeks old, soil grown Arabidopsis lyrata ssp. lyrata plants were treated either with 250 or 500 mM NaCl solution by flush flooding, while the control group was watered.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Triazol extraction of total RNA and subsequent purification with Rneasy mini spin columns
|
| Label |
Cy3
|
| Label protocol |
Cy3 labelling by using the one colour low-amp quick amplification labeling kit (Agilent technologies)
|
| |
|
| Hybridization protocol |
As per Agilent recommedations
|
| Scan protocol |
Data were extracted by using an Agilent scanner and an Agilent Feature Extraction program.
|
| Description |
Gene expression of early and late time points after moderate and high conc. of NaCl treatments
|
| Data processing |
Background corrected and multiplicatively de-trended hybridization signals were imported to GeneSpring (G3784AA, version 2011) for log2 transformation and data normalization.
|
| |
|
| Submission date |
Apr 11, 2016 |
| Last update date |
Sep 14, 2016 |
| Contact name |
Malay Das |
| E-mail(s) |
malay.dbs@presiuniv.ac.in
|
| Organization name |
Presidency University
|
| Department |
Biological Sciences
|
| Lab |
Plant Molecular Biology
|
| Street address |
86/1 College Street
|
| City |
Kolkata |
| State/province |
West Bengal |
| ZIP/Postal code |
700073 |
| Country |
India |
| |
|
| Platform ID |
GPL21706 |
| Series (1) |
| GSE80115 |
A. lyrata transcriptomic responses against salt stress |
|
