|
| Status |
Public on Jul 18, 2007 |
| Title |
Aldosterone-producing adenoma (APA15) |
| Sample type |
RNA |
| |
|
| Source name |
Aldosterone-producing adenoma
|
| Organism |
Homo sapiens |
| Characteristics |
Genotype: Homo sapiens
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The tissue was pulverized in liquid nitrogen to a powder. Total RNA was extracted using TRIzol Reagent (Invitrogen, Carlsbad, CA). The purity and integrity of the RNA were assessed by the Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA) and its quantity was determined by NanoDrop spectrophotometer (NanoDrop Technologies, Wilmington, DE).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001,
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Affymetrix Human Genome U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from aldosterone-producing adenoma
|
| Data processing |
The data were analyzed with GeneSpring 7.3 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1.
|
| |
|
| Submission date |
Jul 18, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Ping Ye |
| E-mail(s) |
pye@mcg.edu
|
| Phone |
706 721 8779
|
| Fax |
706 721 8360
|
| Organization name |
Medical College of Georgia
|
| Department |
Physiology
|
| Lab |
CA 3093
|
| Street address |
1120 15th Street
|
| City |
Augusta |
| State/province |
GA |
| ZIP/Postal code |
30912 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE8514 |
Expression data from normal adrenal gland and aldosterone-producing adenoma |
|
| Relations |
| Reanalyzed by |
GSE64985 |
| Reanalyzed by |
GSE119087 |
