|
| Status |
Public on Sep 07, 2007 |
| Title |
mixed stage non-polyadenylated RNA (fishout of polyadenylated RNA), three replicates |
| Sample type |
other |
| |
|
| Source name |
non-polyadenylated RNA (fishout of polyadenylated RNA) extracted from mix-stage N2 worms total RNA
|
| Organism |
Caenorhabditis elegans |
| Characteristics |
N2 worms, polyA- RNA, Stage: mix
|
| Treatment protocol |
no special treament
|
| Growth protocol |
mixed stage wild type N2 strain worms cultivated at 20C
|
| Extracted molecule |
other |
| Extraction protocol |
RNA was extracted according to the Trizol (Invitrogen) protocol.
|
| Label |
biotin
|
| Label protocol |
Biotinylated dsDNA were prepared according to the standard Affymetrix protocol (GeneChip® Whole Transcript (WT) Double-Stranded Target Assay Manual, 2006, Affymetrix).
|
| |
|
| Hybridization protocol |
Following the protocol of GeneChip® Whole Transcript (WT) Double-Stranded Target Assay Manual, 2006, Affymetrix.
|
| Scan protocol |
GeneChips were scanned using the GeneChip® Scanner 3000 7G.
|
| Description |
non-polyadenylated RNA (fishout of polyadenylated RNA), three biological replicates
|
| Data processing |
The data were analyzed with Affymetrix Tiling analysis software (version 1.0.15) .
|
| |
|
| Submission date |
Jul 20, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Tao Liu |
| E-mail(s) |
tao.liu@roswellpark.org
|
| Phone |
7168451300
|
| Organization name |
Roswell Park Comprehensive Cancer Institute
|
| Department |
Biostatistics and Bioinformatics
|
| Street address |
Elm and Carlton St
|
| City |
Buffalo |
| State/province |
NY |
| ZIP/Postal code |
14263 |
| Country |
USA |
| |
|
| Platform ID |
GPL5634 |
| Series (1) |
| GSE8543 |
Mapping the C. elegans non-coding transcriptome with a whole genome tiling microarray |
|
