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Status |
Public on Feb 02, 2017 |
Title |
Liver_PO_basal_rep9 |
Sample type |
RNA |
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Source name |
Liver tissue derived from C57BL/6NTac mice treated with palm oil
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Organism |
Mus musculus |
Characteristics |
strain/background: C57BL/6NTac genotype/variation: WT age: 14 weeks gender: male tissue: liver treatment: palm oil hyperinsulinemic-euglycemic clamp: no
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Treatment protocol |
As described in the paper. Briefly, mice were fasted for 10 h and were given 2 g/kg BM of PO (Landkrone, Germany) or water (VCL) per gavage. Lateral tail vein blood samples were obtained prior to treatment and 2 hours after. 6 hours after treatment, mice were euthanized with isoflurane and a vena cava-blood sample was collected, centrifuged at 4 °C, and plasma aliquots were immediately frozen in liquid nitrogen. Liver was dissected and immediately snap frozen in liquid nitrogen. For mice undergoing an hyperinsulinemic-euglycemic clamp, a permanent jugular vein catheter was placed in male C57BL/6NTac mice, while under ketamine/xylazine-anesthesia. 6-7 days later, mice were fasted for 10 hours, then received 2g/ kg BM PO or VCL via gavage. 6 hours later, unrestrained, conscious mice underwent euglycemic hyperinsulinemic clamp. After 110 minutes of primed-continuous [3-3H]glucose infusion (1.85 kBq/min), a blood sample was collected to determine plasma insulin, glucose and [3-3H] glucose concentrations for calculating basal EGP. A [3-3H]glucose infusion (3.7 kBq/min) containing insulin (15 pmol/kg·min-1; HumulinR, Lilly, USA) was started. Blood glucose concentrations were measured every 10 minutes and target glycemia established by adjusting the GIR. At minute 120, 2-deoxy-D-[1-14C]glucose (370 kBq) was injected IV, to assess rates of tissue specific glucose uptake (Rg). Mice were euthanized by means of an IV ketamine/ xylazine injection. Liver was collected, immediately snap-frozen in liquid nitrogen, and stored at -80°C.
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Growth protocol |
Animals housed under spf conditions.
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Extracted molecule |
total RNA |
Extraction protocol |
miRNeasy Mini (Qiagen) including DNase treatment.
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Label |
biotin
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Label protocol |
Ovation PicoSL WTA System V2 and Encore Biotin Module (Nugen).
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|
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Hybridization protocol |
According to the Affymetrix expression protocol (HWS kit).
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Scan protocol |
According to the Affymetrix expression protocol, Scanner 3000 7G.
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Description |
PO_B _51 Gene expression data from liver tissue derived from C57BL/6NTac mice treated with palm oil.
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Data processing |
RMA probeset summary with standard settings of the Affymetrix Expression Console software (v.1.3.0.187) including median polish and Sketch-Quantile normalisation.
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Submission date |
Apr 13, 2016 |
Last update date |
Feb 03, 2017 |
Contact name |
Johannes Beckers |
E-mail(s) |
johannes.beckers@helmholtz-munich.de
|
Organization name |
Helmholtz Zentrum Muenchen
|
Department |
Institute of Experimental Genetics
|
Street address |
Ingolstaedter Landstr. 1
|
City |
Neuherberg |
ZIP/Postal code |
85764 |
Country |
Germany |
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|
Platform ID |
GPL17400 |
Series (1) |
GSE80259 |
Acute dietary fat intake initiates alterations in energy metabolism and insulin resistance |
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