|
| Status |
Public on Feb 02, 2017 |
| Title |
Liver_PO_Clamp_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Liver tissue derived from C57BL/6NTac mice treated with palm oil and undergoing a hyperinsulinemic-euglycemic clamp
|
| Organism |
Mus musculus |
| Characteristics |
strain/background: C57BL/6NTac
genotype/variation: WT
age: 14 weeks
gender: male
tissue: liver
treatment: palm oil
hyperinsulinemic-euglycemic clamp: yes
|
| Treatment protocol |
As described in the paper. Briefly, mice were fasted for 10 h and were given 2 g/kg BM of PO (Landkrone, Germany) or water (VCL) per gavage. Lateral tail vein blood samples were obtained prior to treatment and 2 hours after. 6 hours after treatment, mice were euthanized with isoflurane and a vena cava-blood sample was collected, centrifuged at 4 °C, and plasma aliquots were immediately frozen in liquid nitrogen. Liver was dissected and immediately snap frozen in liquid nitrogen. For mice undergoing an hyperinsulinemic-euglycemic clamp, a permanent jugular vein catheter was placed in male C57BL/6NTac mice, while under ketamine/xylazine-anesthesia. 6-7 days later, mice were fasted for 10 hours, then received 2g/ kg BM PO or VCL via gavage. 6 hours later, unrestrained, conscious mice underwent euglycemic hyperinsulinemic clamp.
After 110 minutes of primed-continuous [3-3H]glucose infusion (1.85 kBq/min), a blood sample was collected to determine plasma insulin, glucose and [3-3H] glucose concentrations for calculating basal EGP. A [3-3H]glucose infusion (3.7 kBq/min) containing insulin (15 pmol/kg·min-1; HumulinR, Lilly, USA) was started. Blood glucose concentrations were measured every 10 minutes and target glycemia established by adjusting the GIR. At minute 120, 2-deoxy-D-[1-14C]glucose (370 kBq) was injected IV, to assess rates of tissue specific glucose uptake (Rg). Mice were euthanized by means of an IV ketamine/ xylazine injection. Liver was collected, immediately snap-frozen in liquid nitrogen, and stored at -80°C.
|
| Growth protocol |
Animals housed under spf conditions.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
miRNeasy Mini (Qiagen) including DNase treatment.
|
| Label |
biotin
|
| Label protocol |
Ovation PicoSL WTA System V2 and Encore Biotin Module (Nugen).
|
| |
|
| Hybridization protocol |
According to the Affymetrix expression protocol (HWS kit).
|
| Scan protocol |
According to the Affymetrix expression protocol, Scanner 3000 7G.
|
| Description |
PO_CL_19
Gene expression data from liver tissue derived from C57BL/6NTac mice treated with palm oil and undergoing a hyperinsulinemic-euglycemic clamp.
|
| Data processing |
RMA probeset summary with standard settings of the Affymetrix Expression Console software (v.1.3.0.187) including median polish and Sketch-Quantile normalisation.
|
| |
|
| Submission date |
Apr 13, 2016 |
| Last update date |
Feb 03, 2017 |
| Contact name |
Johannes Beckers |
| E-mail(s) |
johannes.beckers@helmholtz-munich.de
|
| Organization name |
Helmholtz Zentrum Muenchen
|
| Department |
Institute of Experimental Genetics
|
| Street address |
Ingolstaedter Landstr. 1
|
| City |
Neuherberg |
| ZIP/Postal code |
85764 |
| Country |
Germany |
| |
|
| Platform ID |
GPL17400 |
| Series (1) |
| GSE80259 |
Acute dietary fat intake initiates alterations in energy metabolism and insulin resistance |
|
