|
| Status |
Public on Jun 03, 2016 |
| Title |
skin_12weeks_KO_2_miRNA |
| Sample type |
RNA |
| |
|
| Source name |
dorsal skin, 12 weeks UV irradiated, KO(Ppard-/-)
|
| Organism |
Mus musculus |
| Characteristics |
background strain: SKH1-C57/Bl6-SV129
tissue: skin
uv irradiation: 12_weeks_UV
genotype: KO(Ppard-/-)
|
| Treatment protocol |
Irradiated mice: 70mJ/cm2 of UVB three times a week
|
| Growth protocol |
Mice in standard colony, with water and food ad libitum
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA containing the small RNA fraction was isolated using TRIzol reagent (Invitrogen).
|
| Label |
Cy3
|
| Label protocol |
Each sample was prepared according to the Agilent’s miRNA Microarray System protocol. 100 ng of total RNA was dephosphorylated with calf intestine alkaline phosphatase (GE Healthcare Europe GmbH, Otelfingen, Switzerland), denatured with dimethyl sulfoxide and labeled with pCp-Cy3 using T4 RNA ligase (GE Healthcare Europe GmbH, Otelfingen, Switzerland).
|
| |
|
| Hybridization protocol |
The labeled RNA was hybridized to Agilent mouse miRNA microarrays for 20 hours at 55°C with rotation.
|
| Scan protocol |
The arrays were scanned with an Agilent Technologies Scanner G2505B with high dynamic range settings as specified by the manufacturer.
|
| Description |
KO.103
|
| Data processing |
Agilent Feature Extraction Software (version 9.5.3.1) was used to extract the data and quantile normalization was applied to the Total Gene Signal intensity values
|
| |
|
| Submission date |
Apr 19, 2016 |
| Last update date |
Jun 03, 2016 |
| Contact name |
Mark Ibberson |
| Organization name |
SIB Swiss Institute of Bioinformatics
|
| Department |
Vital-IT
|
| Street address |
Genopode building
|
| City |
Lausanne |
| ZIP/Postal code |
CH-1015 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL8824 |
| Series (2) |
| GSE80428 |
Identification of a novel PPARβ/δ / miR-21-3p axis in UV-induced skin inflammation [mouse miRNA] |
| GSE80431 |
Identification of a novel PPARβ/δ / miR-21-3p axis in UV-induced skin inflammation |
|
