|
| Status |
Public on Jun 20, 2016 |
| Title |
Torso_RG_blue gut_-18hr_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Drosophila melanogaster phm22_Torso-RNAi ring gland 18hr PPF
|
| Organism |
Drosophila melanogaster |
| Characteristics |
genotype: RNAi knockdown of Torso
phenotype: developmental delay
accession: Ring gland, late third instar
|
| Treatment protocol |
Genotypes: Samples 1-24: w1118. Samples 25-30: phantom-Gal4/w1118 (control cross). Samples 31-36: phantom-Gal4/RasV12 (constitutively active form of Ras. Samples 37-42: phantom-Gal4/Torso-RNAi.
|
| Growth protocol |
Drosophila larvae were reared in the dark on yeast paste at 25°C. (Ou et al., PLoS Biology, 2011)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using TRIzol and the RNeasy Mini kit (Qiagen Inc., Valencia, CA, USA) and the RNAprotect reagent (Qiagen). RNA quality and concentration was determined by analysis with a NanoDrop-1000 spectrophotometer and an Agilent 2100 bioanalyzer at the Molecular Biology Service Unit (University of Alberta, Edmonton, Canada).
|
| Label |
Cy3
|
| Label protocol |
Nucleic acid labeling was carried out by authors, following the standard operating protocol provided by Roche one-colour DNA labeling kit (Cat. No. 05223555001).
|
| |
|
| Hybridization protocol |
Hybridization was performed by the Alberta Transplant Applied Genomics Centre, AB, Canada, following the standard protocol.
|
| Scan protocol |
Scanning was performed by the Alberta Transplant Applied Genomics Centre, AB, Canada, following the standard operating protocol.
|
| Description |
This sample is of Torso-RNAi ring gland of 18hours PPF. It is the second of three wild-type biological replicates used in this experiment.
|
| Data processing |
The raw data (.pair file) was analyzed with Arraystar 4.0 (based on RMA: Robust Multi-Array Analysis; Irizarry et al. Biostatistics 4(2):249). Background correction was carried out with the NimbleScan software package, version 2.5 (Roche NimbleGen, Inc.).
|
| |
|
| Submission date |
Apr 20, 2016 |
| Last update date |
Jun 20, 2016 |
| Contact name |
Kirst King-Jones |
| E-mail(s) |
kingjones@ualberta.ca
|
| Organization name |
University of Alberta
|
| Department |
Biological Sciences
|
| Lab |
Kirst King-Jones laboratory
|
| Street address |
11455 saskatchewan driver
|
| City |
Edmonton |
| State/province |
Alberta |
| ZIP/Postal code |
T6G2E9 |
| Country |
Canada |
| |
|
| Platform ID |
GPL15486 |
| Series (2) |
| GSE80475 |
Genome-wide transcriptional analysis of Drosophila ring gland |
| GSE80485 |
The insect prothoracic gland as a model for steroid hormone biosynthesis and regulation |
|
