|
| Status |
Public on Aug 02, 2007 |
| Title |
1st MPSS library produced from activated CD4+ T cell clone 29 |
| Sample type |
MPSS |
| |
|
| Source name |
human CD4+ T cell clone 29
|
| Organism |
Homo sapiens |
| Characteristics |
CD4+ T cell clone 29 derived from PBMC from subject
Immunogen: MVA virus containing HIV-1 gag gene
Peptide Specificity: KRWIILGLNKVIRMY
TCR usage: Vbeta17
|
| Treatment protocol |
T cells were activated with beads coated in monoclonal antibodies
specific for CD3 and CD28.
|
| Growth protocol |
The clone was established in culture with IL-7 and the HIV-1 gag peptides KRWIILGLNKVIRMY and GEIYKRWIILGLNKI (but later shown to be specific for the former)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from T cells using Trizol according to standard
methods and sent to Lynx therpeutics for the generation and sequencing of an MPSS library.
|
| |
|
| Description |
1st MPSS library produced from a single sample of RNA from an activated CD4+ T-cell clone.
|
| Data processing |
Data file includes simply the tags identified and their counts in the library. The supplementary data file contains the equivalent data after fewer cycles of the sequencing process (i.e. 17 base and 14 base MPSS tags) as provided by Lynx therpeutics.
|
| |
|
| Submission date |
Jul 27, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Edward J Evans |
| E-mail(s) |
edward.evans@ndm.ox.ac.uk
|
| URL |
http://www.t-cellbiology.org
|
| Organization name |
University of Oxford
|
| Department |
Nuffield Dept. Clinical Medicine
|
| Lab |
T-cell Biology Group
|
| Street address |
HIU, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital
|
| City |
Oxford |
| State/province |
Oxfordshire |
| ZIP/Postal code |
OX3 9DS |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL5678 |
| Series (1) |
| GSE8612 |
SAGE and MPSS libraries from activated CD4+ T cell clone 29 |
|
