|
| Status |
Public on Aug 02, 2007 |
| Title |
2nd MPSS library produced from activated CD4+ T cell clone 29 |
| Sample type |
MPSS |
| |
|
| Source name |
human CD4+ T cell clone 29
|
| Organism |
Homo sapiens |
| Characteristics |
CD4+ T cell clone 29 derived from PBMC from subject
Immunogen: MVA virus containing HIV-1 gag gene
Peptide Specificity: KRWIILGLNKVIRMY
TCR usage: Vbeta17
|
| Treatment protocol |
T cells were activated with beads coated in monoclonal antibodies
specific for CD3 and CD28.
|
| Growth protocol |
The clone was established in culture with IL-7 and the HIV-1 gag peptides KRWIILGLNKVIRMY and GEIYKRWIILGLNKI (but later shown to be specific for the former)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from T cells using Trizol according to standard
methods and sent to Lynx therpeutics for the generation and sequencing of an MPSS library.
|
| |
|
| Description |
2nd MPSS library produced from a single sample of RNA from an activated CD4+ T-cell clone.
|
| Data processing |
Data file includes simply the tags identified and their counts in the library
|
| |
|
| Submission date |
Jul 27, 2007 |
| Last update date |
Aug 01, 2007 |
| Contact name |
Edward J Evans |
| E-mail(s) |
edward.evans@ndm.ox.ac.uk
|
| URL |
http://www.t-cellbiology.org
|
| Organization name |
University of Oxford
|
| Department |
Nuffield Dept. Clinical Medicine
|
| Lab |
T-cell Biology Group
|
| Street address |
HIU, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital
|
| City |
Oxford |
| State/province |
Oxfordshire |
| ZIP/Postal code |
OX3 9DS |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL5678 |
| Series (1) |
| GSE8612 |
SAGE and MPSS libraries from activated CD4+ T cell clone 29 |
|
