|
| Status |
Public on Feb 27, 2017 |
| Title |
Total RNA from mycelium of Δdcl2 |
| Sample type |
SRA |
| |
|
| Source name |
Δdcl2-MY
|
| Organism |
Metarhizium robertsii |
| Characteristics |
strain: ARSEF 23
genotype/variation: deltadcl2
developmental stage: asexual
age: 1-day
|
| Growth protocol |
Conidia of WT and Δdcl2 were inoculated into SDAY medium at 1×107 conidia/ml at 25°C. Fungal samples were collected after 1 day (mycelium growth, MY) and 2.5 days (conidiogenesis, CO), respectively
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Metarhizium robertsii wild type and Δdcl2 were ground into powder in liquid nitrogen,Powder was used to isolate total RNAs using Trizol (Invitrogen, USA).
The total RNA samples are first treated with DNase I to degrade any possible DNA contamination. Then the mRNA is enriched by using the oligo(dT) magnetic beads. Mixed with the fragmentation buffer, the mRNA is fragmented into short fragments. Then the first strand of cDNA is synthesized by using random hexamer-primer. Buffer, dNTPs, RNase H and DNA polymerase I are added to synthesize the second strand. The double strand cDNA is purified with magnetic beads. End reparation and 3’-end single nucleotide A (adenine) addition is then performed. Finally, sequencing adaptors are ligated to the fragments. The fragments are enriched by PCR amplification. During the QC step, Agilent 2100 Bioanaylzer and ABI StepOnePlus Real-Time PCR System are used to qualify and quantify of the sample library. The library products are ready for sequencing via Illumina HiSeqTM 2000.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
quality control on clean data through drawing base composition chart and quality distribution chart.
|
| |
|
| Submission date |
Apr 27, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Yulong Wang |
| E-mail(s) |
yulong4444@126.com
|
| Organization name |
Anhui Agricultural University
|
| Lab |
Anhui Provincial Key Laboratory of Microbial Pest Control
|
| Street address |
130 West Changjiang Road
|
| City |
Hefei |
| ZIP/Postal code |
230036 |
| Country |
China |
| |
|
| Platform ID |
GPL16039 |
| Series (1) |
| GSE80731 |
Mycelium growth and conidiogenesis of Metarhizium robertsii |
|
| Relations |
| BioSample |
SAMN04909614 |
| SRA |
SRX1733796 |
