|
| Status |
Public on Apr 28, 2017 |
| Title |
Male_Achl_RNAi_4 |
| Sample type |
SRA |
| |
|
| Source name |
heads
|
| Organism |
Drosophila melanogaster |
| Characteristics |
tissue: heads
age: 3-5 day old
develpomental stage: adult
|
| Growth protocol |
3-5 day old adult flies were maintained on standard food at 25 degrees under a 12hr:12hr light:dark condition and samples were collected at ZT2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
fly heads were manually dissected in PBS and homogenized in Ttizol (Life Technologies), total RNAs were collected using Phase lock gels (5Prime) and RNeasy mini kit (Qiagen)
libraries were prepared with 200ng total RNA per sample using TruSeq Stranded mRNA LT prep kit (Illumina)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina MiSeq |
| |
|
| Data processing |
RUM (Grant et al. 2011) was used to align raw reads to the reference Drosophila genome and transcriptome using the following parameters: “--strand-specific --variable-length-reads --bowtie-nu-limit 10 --nu-limit 10”.
Genome_build: dm3
|
| |
|
| Submission date |
Apr 28, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Jiajia Li |
| E-mail(s) |
jli.umsl@gmail.com
|
| Organization name |
University of Missouri-St. Louis
|
| Department |
Biology
|
| Lab |
Hughes Lab
|
| Street address |
435 Research Building, One University Boulevard
|
| City |
St. Louis |
| State/province |
Missouri |
| ZIP/Postal code |
63121 |
| Country |
USA |
| |
|
| Platform ID |
GPL16479 |
| Series (1) |
| GSE80738 |
Achilles is a circadian clock controlled gene that regulates innate immune function in Drosophila |
|
| Relations |
| Reanalyzed by |
GSM3278935 |
| BioSample |
SAMN04910102 |
| SRA |
SRX1734347 |
