|
| Status |
Public on Nov 01, 2016 |
| Title |
YCIII_G |
| Sample type |
RNA |
| |
|
| Source name |
Control / left molar
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Wistar
gender: male
age: 31 days old
tissue: periodontal ligament
|
| Treatment protocol |
periodontal ligament tissue from the first right and left mandibular molars was isolated after tooth extraction
|
| Growth protocol |
4 week old male Wistar rats
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was extracted using the Rneasy Mini Kit (Qiagen)
|
| Label |
biotin
|
| Label protocol |
cRNA were prepared with the Ambion WT expression kit according to manufacturer specifications, with a total RNA starting amount of 200 ng. After cRNA reverse transcription, single-strand cDNA were fragmented and labeled with the Affymetrix GeneChip WT Terminal Labeling Kit.
|
| |
|
| Hybridization protocol |
5.5 ug of biotinilated cDNA were hybridized for 16 hr at 45°C on GeneChips Rat Gene 2.0 ST Arrays. Arrays were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
Arrays were scanned on a 7G affymetrix GeneChip Scanner.
|
| Description |
Gene expression data from the periodontal ligament of 31 days old rat
|
| Data processing |
The data was processed with the Partek Genomics Suite and RMA normalized.
|
| |
|
| Submission date |
Apr 29, 2016 |
| Last update date |
Nov 01, 2016 |
| Contact name |
PIerre Burkhard |
| Organization name |
HUG
|
| Department |
Department of Clinical Neurosciences
|
| Lab |
Division of Neurology
|
| Street address |
4 rue Gabrielle-Perret-Gentil
|
| City |
Geneva 14 |
| ZIP/Postal code |
1211 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL17117 |
| Series (1) |
| GSE80965 |
Increased gene expression in the peridontium of unopposed molars in rats |
|
