|
| Status |
Public on Jun 30, 2008 |
| Title |
B6_Sentrix_P0.5 whole brain_rep 5 |
| Sample type |
RNA |
| |
|
| Source name |
whole brain
|
| Organism |
Mus musculus |
| Characteristics |
Male C57BL/6J mice, P0.5 whole brain, biological replicate 5
|
| Biomaterial provider |
The Jackson Laboratory (Bar Harbor, ME)
|
| Treatment protocol |
.
|
| Growth protocol |
All mice of the same strain and gender were raised in groups of four with food (Teklad 7002; 6%fat; Harlan Teklad, Madison, Wi.) and water freely available. All experiments followed the NIH Guide for the Care and Use of Laboratory Animals and received Institutional Animal Care and Use approval.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was extracted with TRIzol reagent (Invitrogen, Carlsbad, CA) according to the manufacturer’s manual. RNA was treated with DNA-freeTM (Ambion, Austin, Tx.) to remove residual contaminating DNA.
|
| Label |
biotin
|
| Label protocol |
Total RNA was reverse transcribed, then in vitro transcribed to cRNA and labeled with biotin-16-UTP.
|
| |
|
| Hybridization protocol |
The labeled probe was hybridized to Illumina's Sentrix mouse-6 Expression BeadChips (Illumina, San Diego, CA).
|
| Scan protocol |
Illumina's BeadStation 500GX Genetic Analysis System
|
| Description |
B6_Sentrix_P0.5 whole brain_rep 5
|
| Data processing |
The raw image data of Illumina BeadChip arrays were analyzed and normalized using the cubic spline method via the BeadStudio software (Illumina, San Diego, CA).
|
| |
|
| Submission date |
Jul 31, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Sanghyeon Kim |
| E-mail(s) |
Sanghyeon.Kim@UTSouthwestern.edu
|
| Organization name |
UT Southwestern medical center
|
| Street address |
5323 Harry Hines Blvd.
|
| City |
Dallas |
| State/province |
TX |
| ZIP/Postal code |
75390-9070 |
| Country |
USA |
| |
|
| Platform ID |
GPL4055 |
| Series (1) |
| GSE8641 |
Rnf41 is associated with anxiety like behavior, major depression and beta carboline induced seizure |
|
