|
| Status |
Public on Aug 21, 2017 |
| Title |
Hippocampus, Sham (Stroke) at 24h |
| Sample type |
RNA |
| |
|
| Source name |
Hippocampus, sham for stroke, 24h
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain/background: Wistar
tissue: Dissected brain (hippocampus)
gender: male
age: adult
treatment: sham for stroke
time point: 24h
|
| Treatment protocol |
Brain regions (isolated cortices or hippocampi) were extracted and fresh frozen.
|
| Growth protocol |
Animals were maintained, provided with food and water ad libitum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized according to the manufacturer's protocol.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner.
|
| Description |
Stroke_Sham24h
|
| Data processing |
Using Expression Console (Affymetrix), the data were normalized using the RMA algorithm. Ratios of normalized probeset intensity values were calculated for each sample pair and then averaged among the three replicate pairs.
|
| |
|
| Submission date |
May 10, 2016 |
| Last update date |
Aug 21, 2017 |
| Contact name |
Soo Young Kim |
| Organization name |
UC Berkeley
|
| Department |
IB
|
| Lab |
Kaufer Lab
|
| Street address |
430E LKS #3370
|
| City |
Berkeley |
| State/province |
CA |
| ZIP/Postal code |
94720-3370 |
| Country |
USA |
| |
|
| Platform ID |
GPL17117 |
| Series (1) |
| GSE81302 |
Expression data from rat brain following ischemic stroke or undercut |
|
