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Sample GSM2152265 Query DataSets for GSM2152265
Status Public on Oct 23, 2018
Title M-1
Sample type SRA
Source name Mouse M-1 cells
Organism Mus musculus
Characteristics cell line: M-1
cell type: normal mouse M-1 cells
tissue: kidney
Extracted molecule total RNA
Extraction protocol RNA was isolated from siCTRL and siNSUN2 HeLa cells and 3T3-L1 cells, mutiple human other cell lines, mouse other cell lines and tissues using the TRIzol (Invitrogen) reagent by following the company manual. For all samples the RNA integrity was checked using an Agilent Bioanalyzer 2100.
Approximately 2.5 µg of total RNA was then used for library preparation using a TruSeq™ RNA Sample Prep Kit v2 (Illumina, San Diego, CA, USA) according to the manufacturer’s protocol.The libraries were sequenced using HiSeq2000 or HiSeq3000 (Illumina) in paired-read mode, creating reads with a length of 101 bp.
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 3000
Description Transcriptome of normal M-1 cells
Data processing Reads were aligned to the hg19 or mm10 genome assembly using TopHat v2.0.9
For each sample, reads counts of all genes were computed using HTSeq v0.5.3
Genome_build: mm10
Supplementary_files_format_and_content: txt format with read counts
Submission date May 13, 2016
Last update date May 15, 2019
Contact name Bao-Fa Sun
Organization name Beijing Institute of Genomics (BIG) of Chinese Academy of Sciences (CAS)
Street address Da-Tun Road
City Beijing
ZIP/Postal code 100101
Country China
Platform ID GPL21493
Series (1)
GSE74333 Transcriptomics analysis of gene expression in multiple human and mouse cells and tissues
BioSample SAMN04998856
SRA SRX1760408

Supplementary file Size Download File type/resource
GSM2152265_M-1_union.txt.gz 140.6 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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