|
| Status |
Public on Jul 01, 2016 |
| Title |
NRK52_control_ rep.2 |
| Sample type |
RNA |
| |
|
| Source name |
kon
|
| Organism |
Rattus norvegicus |
| Characteristics |
tissue: Renal tissue
|
| Treatment protocol |
Notch1 intracellular domain (NICD) was overexpressed using the pINDUCER21 vector system
|
| Growth protocol |
NRK-52E cells were cultivated in Dulbecco’s modified Eagle medium (DMEM) supplemented with 10% fetal calf serum , 1% antibiotics (Pen/Strep) and L-Glutamine , and were cultured at 37°C and 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
extraction of total RNA was performed using Qiagen Rneasy kit according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Single-stranded cDNA was generated from the amplified cRNA with the WT cDNA Synthesis Kit (Affymetrix) and then fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix).
|
| |
|
| Hybridization protocol |
Samples were hybridized with GeneChip Rat Gene 2.0 ST Arrays (Affymetrix) and scanned at the University Hospital Muenster IFG Microarray Core Facility.
|
| Scan protocol |
Affymetrix Gene ChIP Scanner 3000 7G
|
| Data processing |
Data were processed using Affymetrix Expression Console application. Background correction were applied to probe intensities, gene level expression were summarized from the corrected intensities and then RMA normalized
RaGene-2_0-st.mps
RaGene-1_0-st-v1.r3.mps
|
| |
|
| Submission date |
May 18, 2016 |
| Last update date |
Jul 01, 2016 |
| Contact name |
Bayram Edemir |
| E-mail(s) |
bayram.edemir@uk-halle.de
|
| Phone |
+493455574980
|
| Organization name |
University Hospital Halle (Saale)
|
| Street address |
Ernst-Grube-Str. 20
|
| City |
Halle |
| ZIP/Postal code |
06120 |
| Country |
Germany |
| |
|
| Platform ID |
GPL17117 |
| Series (1) |
| GSE81583 |
Notch induced changes in gene expression in NRK52-cells |
|
