|
| Status |
Public on Oct 28, 2007 |
| Title |
High R:FR 16 degrees |
| Sample type |
RNA |
| |
|
| Source name |
High R:FR, 16 degrees
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
Accession: La-er
Mutant: phyA-2 (WHitelam et al., 1993, Plant Cell 5, 757-768)
Age: 5 days
|
| Biomaterial provider |
Keara Franklin
|
| Treatment protocol |
None
|
| Growth protocol |
Seedlings were grown for 5 days on soil in 100µmolm-2s-1 of white light (R:FR 4.4) at 16 degrees.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from cotyledon (and a small amount of hypocotyl) tissue using RNeasy kits (Qiagen) followed by extraction with acid phenol chloroform and precipitation with ethanol to a concentration > 3mg/ml.
|
| Label |
http://www.affymetrix.com
|
| Label protocol |
Labelling was carried out by the John Innes Centre Genome Laboratory (http://jicgenomelab.co.uk) according to Affymetrix standard protocols (http://www.affymetrix.com).
|
| |
|
| Hybridization protocol |
Hybridization was carried out by the John Innes Centre Genome Laboratory (http://jicgenomelab.co.uk) according to Affymetrix standard protocols (http://www.affymetrix.com).
|
| Scan protocol |
Scanning was carried out by the John Innes Centre Genome Laboratory (http://jicgenomelab.co.uk) according to Affymetrix standard protocols (http://www.affymetrix.com).
|
| Description |
Affymetrix standard protocols.
|
| Data processing |
Microarray suite 5.0 (Affymetrix) was used for image analysis and to determine probe signal levels. Normalization procedures can be found at http://jicgenomelab.co.uk. Data from MAS 5.0 was analysed in GeneSpring software version 5.1 (Silicon Genetics, Redwood City, CA).
|
| |
|
| Submission date |
Aug 10, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Keara Franklin |
| E-mail(s) |
kaf5@le.ac.uk
|
| Organization name |
University of Leicester
|
| Department |
Biology
|
| Lab |
331
|
| Street address |
University Road
|
| City |
Leicester |
| ZIP/Postal code |
LE1 7RH |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE8745 |
Low R:FR treatment at 16 and 22 degrees |
|
| Relations |
| Reanalyzed by |
GSE119083 |
