|
| Status |
Public on May 24, 2016 |
| Title |
0 ng/ml tacrolimus, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
HepG2 cells treated with 0 ng/ml tacrolimus for 24 hr
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: liver cancer cells
|
| Treatment protocol |
HepG2 cells were treated with 0, 5, 20 ng/ml tacrolimus for 24 hrs and RNA was extracted. The expression of mirnas were compared between low-dose tacrolimus group and control, high-dose tacrolimus and control.
|
| Growth protocol |
cells were cultured in DMEM (Life technologies) containing 1.0 g/L of glucose and 10% FBS at 37 °C in a humidified atmosphere of 5% CO2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Microarray profiles were obtained using a human Affymetrix GeneChip miRNA 4.0 Array (Affymetrix Technologies, USA) in different concentrations (0, 5, 20 ng/ml) of TAC treated HepG2 cells.
|
| |
|
| Hybridization protocol |
The procedure was performed according to the manufacturer's instructions
|
| Scan protocol |
GeneChips were scanned using the affymetrix GeneChip2 Scanner 3000 7G.
|
| Description |
Gene expression data from HepG2 cells treated with 0 ng/ml tacrolimus (control group) for 24 hr.
|
| Data processing |
Gene-Cloud of Biotechnology Information (https://www.gcbi.com.cn/gclib/html/index) was used to performed microarray data analysis
|
| |
|
| Submission date |
May 23, 2016 |
| Last update date |
May 24, 2016 |
| Contact name |
Qi Ling |
| E-mail(s) |
lingqilynch@163.com
|
| Phone |
+8657187236567
|
| Organization name |
Zhejiang University
|
| Department |
Suegery
|
| Lab |
transplantation
|
| Street address |
79 Qingchun road
|
| City |
Hangzhou |
| State/province |
Zhejiang |
| ZIP/Postal code |
313000 |
| Country |
China |
| |
|
| Platform ID |
GPL21572 |
| Series (1) |
| GSE81767 |
The impact of tacrolimus on hepatic mirnas |
|
