|
| Status |
Public on Oct 08, 2016 |
| Title |
Msn2A6_0min R2 |
| Sample type |
RNA |
| |
|
| Source name |
S.c. strain BY4741
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
strain: BY4741
estradiol treatment: no
|
| Treatment protocol |
50ml cultures in YPD at 30°C to OD600nm of about 1 before 17-ß-Estradiol was added to a final concentration of 6nM. After 90 minutes cells were harvested and immediately frozen
|
| Growth protocol |
Yeast cells were pre-grown in liquid YPD medium over night. After dilution to OD 0.1 in fresh YPD grown to OD 0.5 were 17ß-Estradiol was added to final concentration of and were harvested after 90 minutes.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were harvested by centrifugation washed and frozen. Extracts were made with bead beating with the phenol chloroform method. RNA was precipitated with ethanol, and dissolved in RNAse free water.
|
| Label |
Cy3
|
| Label protocol |
RNA quality was checked on RNA 6000 Nano chips using a Bioanalyzer 2100 (Agilent Technologies, Palo Alto, CA, USA). Agilent's Low Input Quick Amp Labeling Kit, one-color (Kat. No.: 5190-2305) was used to generate fluorescent cRNA. The amplified cyanine 3-labeled cRNA samples were then purified using Promega's SV Total RNA Isolation System. and hybridized to Agilent Yeast (V2) Gene Expression Microarrays 8x15K. Microarray slides were washed and scanned with an Agilent Scanner, according to the standard protocol of the manufacturer.
|
| |
|
| Hybridization protocol |
The labelled Samples were hybridized to the Microarray slides, according to the standard protocol of the manufacturer.
|
| Scan protocol |
The slides were scanned with an Agilent Technologies Scanner G2505C, according to the standard protocol (GE1_107_Sep09) using grid 016322_D_F_20120509 as supplied by the manufacturer. Information from probe features was extracted from microarray scan images using the Agilent Feature Extraction software v10.7.3.
|
| Data processing |
The raw intensities were imported into Bioconductor and processed with the limma package. Manufacturer control features were removed for further analysis.
|
| |
|
| Submission date |
May 25, 2016 |
| Last update date |
Oct 08, 2016 |
| Contact name |
Christoph Schueller |
| E-mail(s) |
Christoph.schueller@boku.ac.at
|
| Organization name |
University of Natural Resources and Life Sciences, Vienna (BOKU)
|
| Department |
Department of Applied Genetics and Cell Biology
|
| Lab |
Schuller
|
| Street address |
Konrad Lorenz Strasse 24
|
| City |
Tulln |
| ZIP/Postal code |
3430 |
| Country |
Austria |
| |
|
| Platform ID |
GPL16244 |
| Series (1) |
| GSE81890 |
Msn2 controls the yeast metabolic focus |
|
